Language
- Purpose(Empty Backbone) Yeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.
- Depositing LabMarvin Wickens
- PublicationBernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.1016/S1046-2023(02)00015-4.
Backbone
- Vector backboneBluescriptIIKS+
- Backbone size (bp)7887
- Vector typeYeast Expression
- Selectable markersURA3
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Growth Temperature37°C
- Growth Strain(s)DH5alpha
- Copy numberHigh Copy
Depositor Comments
Addgene QC found an indel between SK primer and RPR1 promoter.
Addgene原装质粒#220629