NBT是一种氧化剂，广泛用于同BCIP或BCIP衍生物耦合，用来检测磷酸酶活性。NBT也是脱氢酶和其他氧化酶的底物。因此常被用于评估细胞活性，增值和细胞毒性，也常用于组织化学研究。NBT本身无荧光，溶于水，但是酶促分解后形成深蓝色、高不溶性的沉淀。

NBT (Nitro blue tetrazolium, chloride) is an oxidant and is widely used in conjunction with BCIP or BCIP derivatives to detect phosphatase activity. NBT is also a substrate for dehydrogenate and other oxidizes. Thus, it is often used to assess cell viability, proliferation and cytotoxicity, and is also frequently used in histochemistry studies. The colorless NBT is soluble in water, but forms a dark blue-colored, highly insoluble precipitate after being enzymatically reduced.

• White solid soluble in water and DMSO
• Store at 4°C
• C₄₀H₃₀Cl₂N₁₀O₆
• MW: 817.7