PMA™高效选择和灵敏的检测死细菌中存在的活菌数在许多 实际应用，包括安全检查，食品，饮用水的质量控制，医疗诊 断等方面至关重要。传统使用细菌培养的检测方法，既费时又 不灵敏。建立在实时荧光定量qPCR上的检测，是一个快速而高 度敏感的替代方法。然而普通qPCR方法的分析结果并不能区分 活死细胞。 由 Biotium 开发的新型的DNA 修饰染料-- 叠氮溴化丙锭 （PMA™）克服了该难题。用PMA 预处理的样本用于PCR 分析，为 选择性的检测活细菌提供一个高度敏感和可靠的方式。PMA ™是 一种DNA 高亲和力的光反应染料，染料嵌入双链DNA 暴露在强烈的可见光下形成共价连接，形成化学修饰DNA，不能经PCR扩增( 图1）。由于PMA ™的设计是细胞膜不透性，检测包含活、死细菌 的样本时，只有死细菌由于细胞膜损害，其DNA 敏感的被PMA ™ 修饰。随后可以通过定量PCR 选择性的检测活细菌。 PMA™-定量PCR 技术不仅应用于细菌，还可以用于其他类型 的细胞。 biotium 还提供其他配套产品，包括优越的荧光定量 饱和染料EvaGreen qPCR 和安全高度敏感的核酸凝胶染料GelRed ™和 GelGreen ™。

PMA (propidium monoazide) is a photo-reactive DNA-binding dye used in viability PCR (v-PCR) of microorganisms like bacteria, viruses, and fungi. PMA is available as a 1 mg lyophilized solid or 20 mM solution in water. Also try PMAxx™ (40069), a superior alternative to PMA.

• Selectively detect viable cells using qPCR
• Validated in hundreds of publications
• Dead cell specific dye, binds to DNA
• Covalently attaches to DNA after photoactivation
• Available as 1 mg lyophilized solid or 20 mM solution in water

To learn more about the advantages of determining microbial or cell viability using viability PCR, visit the Viability PCR Technology Page.

Product	Catalog Number	Unit Size	Format
PMA Dye	40013	10 mg	Lyophilized solid
PMA Dye, 20 mM in H2O	40019	100 uL (20 mM in water)	Solution

About PMA

PMA dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to DNA with high affinity. Upon photolysis with visible light, PMA dye becomes covalently attached to DNA. This modified DNA cannot be amplified by PCR. The dye is designed to be cell membrane-impermeable. Thus, in a population of live and dead cells, only dead cells are susceptible to DNA modification due to compromised cell membranes. This unique feature of PMA dye makes it highly useful in selective detection of live bacteria by qPCR.

Since Biotium first developed PMA dye, there have been hundreds of publications on the use of the dye in many sample types including dozens of bacterial strains, biofilms, yeast, fungi, viruses, and eukaryotic cells. It has been used in such applications as food and water safety and environmental testing, and has been used in conjunction with qPCR, NextGen Sequencing (NGS), Sanger sequencing, and Loop-mediated Isothermal Amplification (LAMP).

PMAxx technology is covered by granted and/or pending US and international patents.