Cheetah Taq 是Biotium公司的一种新型化学修饰的热启动 DNA 聚 合酶。和 AmpliTaq Gold 不同的是， Cheetah Taq 在标准热启动 条件下（例如：使用 pH 8-9 Tris 缓冲液并置于94°C），仅需 2 分钟即可完全激活。而另一方面，与 Platinum Taq 等基于抗体技 术的 热启动聚合酶相比，Cheetah Taq 在室温下完全没有活性， 同时也降低了内在的 DNA 污染。此外，总体来说，Cheetah Taq 在 -20°C 和 4°C 时的稳定性都优于 AmpliTaq Gold，这也保证 了产品在低温保存时仍能维持自身的高品质。

Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.

Features

• Hot-start Taq polymerase
• Modified using unique hot-start chemistry
• Absolutely no activity at room temperature
• Fast activation: takes less than 2 minutes of heating to activate
• Very stable during storage

Components

• Cheetah™ Taq, 5 U/uL, 100 uL
  (in buffer containing Tris-HCl pH 9.0, DTT, EDTA, KCl and glycerol)
• 10X Cheetah™ Taq Reaction Buffer (without Mg²⁺), 1.5 mL
• 25 mM MgCl₂, 1.5 mL

Under standard hot-start conditions (i.e., 94°C in pH 8-9 Tris), Cheetah™ Taq regains both the 3′-5′ polymerase and 5′-exonuclease activities within two minutes. Cheetah™ Taq is also superior to antibody-based hot-start DNA polymerases due to its complete suppression of enzyme activity at room temperature and full recovery of enzyme activity following activation.