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| ■ 产品特点 | ||||||||||
| · 室温下操作，可应用于哺乳动物细胞和细菌样品 · 操作简单快速，蛋白质小量纯化5 min即可完成，大量纯化也仅需15-30 min · 操作时间短，有利于蛋白质样品保持活性 · 柱床体积小、带入污染少，与传统的镍离子树脂相比，产物纯度更高 · 一次性使用纯化柱，避免样品之间交叉污染 · 除了部分产品中提供的xTractor Buffer以外，还兼容其他的裂解buffer，通用性强 · 兼容多种添加剂（如β-ME、EDTA、DTT、甘油、TCEP等），可在变性或非变性条件下进行纯化 | ||||||||||
| ■ 各种试剂盒组成 | ||||||||||
| · Capturem™ His-Tagged Purification Miniprep Kit （635710） | ||||||||||
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| · Capturem™ His-Tagged Purification Maxiprep Kit （635713） | ||||||||||
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| · Capturem™ His-Tagged Purification Maxiprep Columns（635715）* | ||||||||||
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| · Capturem™ His-Tagged Purification Maxiprep Columns（635719）* | ||||||||||
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| · Capturem™ His-Tagged Purification 96（635714）* | ||||||||||
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| · Capturem™ His-Tagged Purification Large Volume（635724）* | ||||||||||
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| · Capturem™ His-Tagged Purification 24-Well Plate（635730）* | ||||||||||
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| *注：Capturem™ His-Tagged Purification 24-well、96-well、Maxiprep Columns以及Large Volume中不含buffer，请使用以下组分的buffer： | ||||||||||
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| 参考文献 | ||||||||||
| [1] Stephanie S. Pavlovich, Sean P. Lovett,Galina Koroleva, et al. The Egyptian Rousette Genome Reveals Unexpected Features of Bat Antiviral Immunity[J]. Cell, 2018, 173(5):1098–1100. [2] Sandra Luz Martínez-Hernández, Daniel Cervantes-García, Martín Munoz-Ortega, et al. An anti-amoebic vaccine: generation of the recombinant antigen LC3 from Entamoeba histolytica linked to mutated exotoxin A (PEDIII) via the Pichia pastoris system[J]. Biotechnology Letters, 2017, 39 (8):1149-1157. [3] Min Kong, Fengjuan Wang, Liuying Tian, et al. Functional identification of glutamate cysteine ligase and glutathione synthetase in the marine yeast Rhodosporidium diobovatum[J]. Science of Nature, 2018, 105 (1-2):4. [4] Ariana Kariminejada, Mohammadreza Barzgarb, Bita Bozorgmehra, et al. Novel mutations and a severe neurological phenotype in Sjogren-Larsson syndrome patients from Iran[J]. European Journal of Medical Genetics, 2018, 61 (3):139. [5] Naho Mugita, Takayuki Nambu, Kazuya Takahashi, et al. Proteases, actinidin, papain and trypsin reduce oral biofilm on the tongue in elderly subjects and in vitro[J]. Archives of Oral Biology, 2017, 82:233-240. [6] Xiao-Xiao Cheng, Li-Hong Zhao, Steven J.Klosterman, et al. The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses[J]. Plant Science, 2017, 259:12. |
镍离子快速纯化柱—Capturem™ 膜技术