Column:

Sapphire-C18, 5 µm, 100 Å, 4.6 × 150 mm

Mobile phase:

ACN : 0.05%SDS (containing 0.1%H3PO4) = 48 : 52 (v/v)

Flow rate:

1.0 mL/min

Temperature:

Ambient

Detection:

UV 265 nm

Injection Volume:

10 µL

Sample:

1. Berberine hydrochloride （盐酸小蘗碱）