Flex-T™ HLA-A*01:01 Monomer UVX
Catalog# / Size 280001 / 50 µg
Regulatory Status RUO
Other Names MHC tetramer, HLA tetramer, HLA monomer, MHC monomer, biotinylated HLA monomer
Description CD8 T cells are essential components of the immune system. They function through TCR
recognition of antigens (peptide epitopes) presented by the class I MHC (HLA in humans)
molecules. Thus, TCR recognition of cognate MHC/peptide complex can be utilized for the
detection of antigen-specific CD8 T cells. Due to the intrinsic low affinity of MHC/TCR interaction,
the detection of antigen specific T cells requires enhanced binding avidity by multimerization of
MHC/peptide monomers, ensuring the interaction of multiple MHC/TCR simultaneously. This can
be accomplished by using streptavidin to tetramerize biotinylated MHC class I complexes.
In the absence of proper peptide binding, the HLA class I complex will quickly disassociate. FlexT™ UVX (Ultraviolet Exchange) monomer is designed as a biotinylated α-chain, associated with
the β2-microglobulin chain, and stabilized by a UV-labile peptide. The C-terminus of the α-chain is
site-specifically biotinylated by the enzyme BirA. When the complex (monomer) is irradiated with
UV in the presence of a new peptide with proper affinity, it will result in a net peptide exchange.
The new peptide replaces the pre-loaded UV-labile peptide and forms a new MHC monomer. The
new monomers can be then tetramerized and used for labeling antigen-specific T cells or other
studies.
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Product Details
Verified Reactivity Human
Formulation PBS (pH 7.5) containing 0.5% BSA and protease inhibitors.
Concentration 0.2 mg/ml
Storage & Handling Store frozen (-20 or -80°C); shipped in blue ice. Aliquot upon receipt before freezing; avoid repeated
freeze-thaw cycles. Protect from light.