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Product Description: IgG-CutterTM is a recombinant IgG endoproteinase (Mac/IdeS) from Streptococcus pyogenes. It can specifically cleaves peptide bonds in the hinge region of IgG to form Fc and F(ab’)2 fragments. IgG-CutterTM cleaves all subclasses of human, rabbit, sheep, and monkey IgG with a high efficiency. In addition, IgG-CutterTM can cleave IgG2a and IgG3 of mouse IgG.
Product Source: IgG-CutterTM was produced in E. Coli cells transformed with Mac/IdeS gene. This product is sterile and does not contain any components of animal origin.
Reaction Conditions: 20 mM Tris buffer pH 8.0; incubate at 37°C for at least 30 minutes. Cleavage reaction can also be carried out at room temperature using longer time.
Unit Definition: One unit is defined as the amount of enzyme required to digest >95% of 1 ug of human IgG in 30 minutes at 37°C.
Form: Sterile liquid formulation. 20 mM Tris buffer pH 8.0, 50% Glycerol, and 5 mM b-Mercaptoethanol.
Usage: FOR LABORATORY RESEARCH USE ONLY.