• Cat.No.
• CSC-C0357

• Description
• Established in 1990 from the right axillary lymph node of a 48-year-old woman with metastatic malignant melanoma in the right chest

• Recommended Medium
• 90% RPMI-1640 + 10% h.i. FBS

• Organism
• Human

• Morphology
• adherent fibroblasts, not limited to monolayer growth

• Karyotype
• Human hypotriploid karyotype with 20% polyloidy - 65(61-69)<3n>XXX, -4, -5, -9, -10, -12, +2mar, add(4)(p11), del(6)(p21), del(7)(p14)x2

• Quality Control
• Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
  Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
  Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8

• Storage and Shipping
• Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

• Tissue
• melanoma