RBC Bioscience授权ViVaCell生产的HIT Competent Cells,无需热休克直接转化。HIT感受态细胞操作步骤简单,时间短,冻融稳定性好,并具高效转化率。
HIT 感受态细胞优势:只需使用冰块!
与传统感受态细胞相比:
无需热刺激
10min以内完成转化步骤
真正1步/1管过程
效率高达 108 cfu/ug 质粒
增加冻融耐性
简单的操作过程适合于自动化
操作步骤如下(可点击观看):
采用大肠杆菌DH5α菌株经特殊工艺制备得到的感受态细胞,用于DNA的化学转化。使用pUC19质粒检测,转化效率可达108,-70℃保存6个月转化效率不发生改变。
基因型: F-,φ80lacZΔM15, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17(r-, m+), phoA, supE44, λ-, thi-1, gyrA96
DH5α感受态细胞转化效率:1ⅹ108~5ⅹ108 cfu/μg pUC19
DH5α感受态细胞应用:质粒克隆, TA克隆,蓝白斑筛选,筛选重组质粒, cDNA库创建。
相关产品: JM109感受态细胞,BL21(DE3)感受态细胞,HIT-Blue感受态细胞,涂布玻璃珠
参考文献:
a. Identification and characterization of a novel enzyme related to the synthesis of PUFAs derived from Thraustochytrium aureum ATCC 34304
DH Kang, P Anbu, YS Jeong, BP Chaulagain… - Biotechnology and …, 2010 - Springer
b. Phenotypic and genetic characterization of Erwinia rhapontici isolated from diseased Asian pear fruit trees
SP Thapa, SY Cho, JH Hur, CK Lim - Phytoparasitica, 2012 - Springer
c. Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus
JK Kim, GH An, SH Ahn, YH Moon, YL Cha… - Bioprocess and …, 2012 - Springer
d. Enhanced 2, 3-Butanediol Production in Recombinant Klebsiella pneumoniae via Overexpression of Synthesis-Related Genes KB Rim, JH Park, MK Oh, JW Lee - Journal of Microbiology and …, 2012 - papersearch.net