Rat Glycogen synthase kinase-3 beta(GSK3B) ELISA kit
货号: CSB-EL009963RA
别名: , GSK3beta isoform
规格: 96T
中文价格: 3600
种属: Rat
待测物名称: glycogen synthase kinase 3 beta
缩写: GSK3B
蛋白功能1: Biosynthesis/Metabolism
蛋白功能2: glyconeogenesis and glycometabolism
蛋白功能3: Carbohydrate metabolism
检测范围: 0.156 ng/ml-10 ng/ml
灵敏度: 0.039 ng/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
10
2.241
2.356
2.299
2.147
5
1.544
1.648
1.596
1.444
2.5
1.022
0.992
1.007
0.855
1.25
0.661
0.654
0.658
0.506
0.625
0.423
0.432
0.428
0.276
0.312
0.307
0.315
0.311
0.159
0.156
0.228
0.224
0.226
0.074
0
0.151
0.152
0.152
精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat GSK3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
92
Range %
86-96
1:2
Average %
102
Range %
97-104
1:4
Average %
95
Range %
89-99
1:8
Average %
97
Range %
92-104
回收率:
The recovery of rat GSK3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
89-97
EDTA plasma (n=4)
98
92-102
研究领域: Metabolism
引用文献:
1. , Zhang Q.et al,Toxicology.,2016,347-349:40-6.
2. , Rana A. et al,Clin Exp Pharmacol Physiol,2015,19
Language
大鼠糖原合成酶激酶3β(GSK3B)ELISA kit/ R