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重组人白介素2 /Recombinant Human IL-
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更新 2025-08-12 15:03
 
详细信息
产品名:重组人白介素2 /Recombinant Human IL-2
 
产品号: LT0112
 
产品信息:
IL-2又称为T细胞生长因子(T cell growth factor, TCGF),是引起T细胞增殖最重要的细胞因子。IL-2通过与T细胞表面的IL-2受体(IL-2R)的特异性结合而促使T细胞活化,并进入细胞分裂状态。此外,IL-2还可刺激NK细胞的生长并增强其杀伤能力。IL-2与白介2家族的其他T细胞生长因子通过共用受体可活化和扩增CD4+与CD8+ T细胞。IL-2对T细胞和NK细胞的激活作用通过自分泌和旁分泌方式进行。与T细胞不同,NK细胞不表达α受体亚单位,因此对IL-2亲和力较低。但IL-2能促进NK细胞增殖,增强细胞活性和其他细胞因子的分泌。B细胞也受IL-2激活,从而增殖和分化。
 
别名:T-cell growth factor (TCGF), Aldesleukin
 
英文描述:
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation.  IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response.  IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.  Recombinant Human IL-2 is a 15.5 kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.
 
表达宿主来源:
E.coli
 
氨基酸序列:
MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCL EEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFAQSIISTLT
 
使用建议:
建议使用浓度:20-50ng/ml或500-1000U/ml。
 
保存条件:
4℃保存6个月,-20℃保存一年或-80℃长期保存。
 
纯度&内毒素:
Greater than 95% by SDS-PAGE
gel and HPLC analyses.
Endotoxin Level<0.5EU/μg


The purity of rhIL2 was determined by SDS-PAGE of reduced rhIL2 and staining overnight with Coomassie Blue.
 
生物活性:
The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.