去泛素化酶（DUBs）,是一类数量很大的蛋白酶类家族。它主要通过水解泛素羧基末端的酯键、肽键或异肽键，将泛素分子特异性的从链接有泛素的蛋白质或者前体蛋白水解下来。人类基因组编码近100种去泛素酶，使得它们成为泛素系统酶中最大家族。在人类中的去泛素化酶基因，可分为两大类：半胱氨酸蛋白酶家族和金属蛋白酶家族。半胱氨酸蛋白酶家族包含一些泛素特异型加工蛋白酶（USPs），泛素羧基末端端水解酶（UCHs），Machado-Josephin结构域蛋白酶（MJDS）和卵巢肿瘤相关蛋白酶（OTU）。金属蛋白酶家族只包含MPN(+)/JAMM蛋白酶家族。 

       去泛素化酶在泛素化途径中扮演重要的角色，在泛素-蛋白酶系统中已泛素化的蛋白底物，通过裂解切断泛素链与底物蛋白之间的连接及泛素化链之间的连接，使泛素分子从蛋白质底物中脱离出来。 



      去泛素化酶基因家族一共有100多个，本公司现已将接近200个基因克隆构建至慢病毒载体，新的基因持续克隆更新中。
      
     载体骨架是慢病毒载体：


表. 去泛素化酶基因载体列表。
 

The Human DUB Superfamily Tree
Family	编号	Name	length/bp	NCBI Reference Sequence
USP  FAMILY	L1	USP15	2995	NM_001252078.1	NP_001239007.1
	L2	USP4	2942	NM_003363.3	NP_003354.2
	L3	USP11	2942	NM_004651.3	NP_004642.2
	L4	USP32	4897	NM_032582.3	NP_115971.2
	L5	USP6	4293	NM_001304284.1	NP_001291213.1
	L6	USP8	3414	NM_005154.4	NP_005145.3
	L7	USP19	4023	NM_006677.2	NP_006668.1
	L8	USP21	1728	NM_001014443.2	NP_001014443.1
	L9	USP2	1850	NM_004205.4	NP_004196.4
	L10	USP31	4128	NM_020718.3	NP_065769.3
	L11	USP43	3430	NM_153210.4	NP_694942.3
	L12	USP50*	1023	NM_203494.4	NP_987090.2
	L13	USP39*	1728	NM_001256725.1	NP_001243654.1
	L14	USP20	2792	NM_001008563.4	NP_001008563.2
	L15	USP33	2878	NM_015017.4	NP_055832.3
	L16	USP16	2515	NM_001032410.1	NP_001027582.1
	L17	USP45	2487	NM_001080481.1	NP_001073950.1
	L18	USP3	1591	NM_006537.3	NP_006528.2
	L19	USP51	2173	NM_201286.3	NP_958443.1
	L20	USP22	1606	NM_015276.1	NP_056091.1
	L21	USP27X	1340	NM_001145073.2	NP_001138545.1
	L22	USP10	2438	NM_005153.2	NP_005144.2
	L23	USP17	1616	NM_201402.2	NP_958804.2
	L24	USP36	3430	NM_001321291.1	NP_001308220.1
	L25	USP42	3981	NM_032172.2	NP_115548.1
	L26	USP44	2176	NM_001042403.2	NP_001035862.1
	L27	USP49	2103	NM_001286554.1	NP_001273483.1
	L28	USP12	1132	NM_182488.3	NP_872294.2
	L29	USP46	1120	NM_022832.3	NP_073743.2
	L30	USP35	3107	NM_020798.3	NP_065849.1
	L31	USP38	3181	NM_032557.6	NP_115946.2
	L32	USP37	2988	NM_020935.2	NP_065986.2
	L33	USP26	2787	NM_031907.1	NP_114113.1
	L34	USP29	2815	NM_020903.2	NP_065954.1
	L35	USP13	2635	NM_003940.2	NP_003931.2
	L36	USP5	2619	NM_001098536.1	NP_001092006.1
	L37	CYLD	2921	NM_015247.2	NP_056062.1
	L38	USP48	3162	NM_032236.7	NP_115612.4
	L39	USP14	1512	NM_005151.3	NP_005142.1
	L40	USP47	4199	NM_001330208.1	NP_001317137.1
	L41	USP40	3772	NM_018218.2	NP_060688.1
	L42	USP7	3365	NM_003470.2	NP_003461.2
	L43	USP30	1579	NM_032663.4	NP_116052.2
	L44	USP18	1138	NM_017414.3	NP_059110.2
	L45	USP41	1094	XM_017029164.1	XP_016884653.1
	L46	USP25	3318	NM_001283042.2	NP_001269971.1
	L47	USP28	3287	NM_020886.3	NP_065937.1
	L48	USP34	10818	NM_014709.3	NP_055524.3
	L49	USP24	7994	NM_015306.2	NP_056121.2
	L50	USP9X	7841	NM_001039590.2	NP_001034679.2
	L51	USP9Y	7795	NM_004654.3	NP_004645.2
	L52	USP1	2397	NM_003368.4	NP_003359.3
	L53	USP52*	3661	NM_001127460.2	NP_001120932.1
	L54	USPL1	3333	NM_005800.4	NP_005791.3
	L55	USP53*	3275	NM_019050.2	NP_061923.2
	L56	USP54*	5139	NM_152586.3	NP_689799.3
UCH  FAMILY	L57	UCHL1	683	NM_004181.4	NP_004172.2
	L58	UCHL3	705	NM_006002.4	NP_005993.1
	L59	UCHL5	1007	NM_015984.4	NP_057068.1
	L60	BAP1	2226	NM_004656.3	NP_004647.1
JOSEPHIN  FAMILY	L61	ATXN3	1104	NM_004993.5	NP_004984.2
	L62	ATXN3L	1086	NM_001135995.1	NP_001129467.1
	L63	JOSD1	619	NM_001360235.1	NP_001347164.1
	L64	JOSD2	576	NM_001270639.1	NP_001257568.1
OTU  FAMILY	L65	OTUD4	3400	XM_005263079.4	XP_005263136.1
	L66	HIN1L**	1351	GenBank: BN000160.1
	L67	OTU1	827	NM_017670.2	NP_060140.2
	L68	OTUD1	1470	NM_001145373.2	NP_001138845.1
	L69	OTUD5	1744	NM_017602.3	NP_060072.1
	L70	OTUD3	1216	NM_015207.1	NP_056022.1
	L71	OTUD6A	881	NM_207320.2	NP_997203.1
	L72	OTUD6B	896	NM_016023.4	NP_057107.4
	L73	OTUB1	829	NM_017670.2	NP_060140.2
	L74	OTUB2	716	NM_023112.3	NP_075601.1
	L75	VCPIP	3722	NM_025054.4	NP_079330.2
	L76	TRABID	2157	NM_017580.2	NP_060050.2
	L77	A20	2407	NM_001270508.1	NP_001257437.1
	L78	Cezanne2	2821	NM_130901.2	NP_570971.1
	L79	Cezanne	2568	NM_020205.3	NP_064590.2
	L80	FAM105*	1088	NM_019018.2	NP_061891.1
	L81	OTULIN	1076	NM_138348.5	NP_612357.4
JAMM/MPN+ FAMILY	L82	CSN5	1020	NM_006837.2	NP_006828.2
	L83	POH1	947	NM_005805.5	NP_005796.1
	L84	BRCC3	966	NM_024332.3	NP_077308.1
	L85	MPND	1439	NM_032868.5	NP_116257.2
	L86	MYSM1	2528	NM_001085487.2	NP_001078956.1
	L87	EIF3H*	1076	NM_003756.2	NP_003747.1
	L88	CSN6*	999	NM_006833.4	NP_006824.2
	L89	PSMD7*	991	NM_002811.4	NP_002802.2
	L90	EIF3F*	1091	NM_003754.2	NP_003745.1
	L91	AMSH	1294	NM_001353967.1	NP_001340896.1
	L92	AMSH-LP	1329	NM_020799.3	NP_065850.1
	L93	PRPF8*	7124	NM_006445.3	NP_006436.3