酶联免疫法测定，用于定量测定血浆和血清中的钙视网膜蛋白。仅供科研使用。
Enzyme Immuno Assay for the Quantitative Determination of Calretinin in Plasma and Serum

临床领域：肿瘤学、癌症筛查、职业病
Clinical Fields：Oncology, Cancer screening, Occupational diseases

疾病：间皮瘤（中国是间皮瘤死亡人数最多的国家。据估计，2017年中国间皮瘤死亡人数超过1万人[1]）
Diseases：Mesothelioma（China is the leading country in terms of mesothelioma death. The estimated number of mesothelioma deaths in China in 2017 was more than 10,000[1]）

规格：微孔板（12 x 8），预包被多克隆钙视网膜蛋白抗体
Format：Microtiter plate (12 x 8 determinations, single break apart wells), coated with polyclonal Calretinin antibodies

样品量：15μl患者血清或血浆
Sample volume: 15 μl patient serum or plasma

样品制备：室温下60分钟振荡
Sample preparation: 60 min shaking at room temperature
ELISA：室温下4.5小时培养，450nm下ELISA读板机的测量值（参考值620nm）
ELISA: 4.5 hours incubation at room temperature
Measurement in ELISA reader at 450 nm (620 nm reference)
6个标准品，范围：0.25 ng/ml - 4.0ng/ml
6 standards with range: 0.25 ng/ml - 4.0 ng/ml
2个试剂盒质控品，冻干
2 kit controls, lyophilized
酶标记抗体浓缩200x，洗涤液浓缩50x
Enzyme conjugate 200x concentrated, wash buffer 50x concentrated
所有其他试剂可立即使用
All other reagents ready for use

特性：灵敏度59%，特异性95%
Specificity：95 % Specificity at 59 % Sensitivity

特性：适用于大批量血清或血浆样品测试，经过大型病例对照研究验证，参与前瞻性研究

Characteristics：Suitable for testing large series of serum or plasma samples
Validated in larger case-control studies
Involved in prospective studies

参考文献：[1]Furuya S, Chimed-Ochir O, Takahashi K, et al. Global Asbestos Disaster[J]. International Journal of Environmental Research & Public Health, 2018, 15(5):1000-.