蛋白酶抑制剂混合液(100×，不含EDTA)
Protease Inhibitor Cocktail(100×，EDTA-free)
本产品冰袋运输；保存于-20℃，保质期12个月。

货号规格
goodGRF101(100×，DMSO溶液)    1 mL

产品特点
gd高效全面——多达9种的高效抑制成分，提供比同类产品(一般含6种抑制剂)更为全面的蛋白保护作用。

产品简介
good本品是一种广谱蛋白酶抑制剂，全面抑制Serine Proteases，Cysteine Proteases，Aspartic Acid-proteases，Aminopeptidases和Metalloproteases等细胞内所含的蛋白酶。

成分说明
good含 AEBSF ， Aprotinin ， Bestatin ， E-64 ， Leupeptin ， Pepstatin A ， Phosphoramidon ，  Benzamidine HCl ， 1,10-phenanthroline 等9种高效蛋白酶抑制剂。

使用说明

good1. 本品可用于各种蛋白质含量或其活性分析实验(Western Blot，Co-IP，pull-down，IF，IHC等)，保护蛋白质免受蛋白酶的降解； good2. 将本品以1:100(体积比)的稀释比加入到目标溶液中(如细胞裂解液，蛋白提取液等)。

注意事项

good1. 本品不含EDTA，如需使用时，需另行添加。当与磷酸酶抑制剂混合液(GRF102)联用时，建议不加EDTA，EDTA会严重影响磷酸酶抑制剂混合液中某些成分的抑制效果； good2. 本品为高浓度溶液，长期存放可能会出现微量晶体状沉淀，此为正常现象，不影响实验结果； good3. 不同来源的样品中蛋白酶含量各不相同，1:100为推荐的常规剂量。实际使用中，如发现蛋白酶含量较高，可适当提高蛋白酶抑制剂的使用浓度； good4. 为了您的安全和健康，请穿实验服并戴一次性手套操作； good5. 本产品仅限科研使用。
  相关论文
good1. Huang, Y., Chen, S., Wu, S., Dong, G., & Sheng, C. (2020). Evodiamine-inspired dual inhibitors of histone deacetylase 1 (HDAC1) and topoisomerase 2 (TOP2) with potent antitumor activity. Acta Pharmaceutica Sinica B, 10(7), 1294-1308. （IF 11.413）
good2. Wang F, Li Z, Zhou J, et al. SIRT1 regulates the phosphorylation and degradation of P27 by deacetylating CDK2 to promote T-cell acute lymphoblastic leukemia progression[J]. Journal of Experimental & Clinical Cancer Research, 2021, 40(1): 1-16.（IF 11.161)
good3. Jiang Z, Wang Y, Li Z, et al. The m6A mRNA demethylase FTO in granulosa cells retards FOS-dependent ovarian aging[J]. Cell death & disease, 2021, 12(8): 1-10.（IF 8.469）
good4. Li J, Sun Z, Luo G, et al. Quercetin attenuates trauma-induced heterotopic ossification by tuning immune cell infiltration and related inflammatory insult[J]. Frontiers in immunology, 2021, 12: 1946.（IF 7.561）
good5. Zhu Y, Tang X, Xu Y, et al. RNASE2 Mediates Age-Associated B Cell Expansion Through Monocyte Derived IL-10 in Patients With Systemic Lupus Erythematosus[J]. Frontiers in immunology, 2022, 13: 752189-752189.（IF 7.561）
good6. Cheng, J., Li, Y., Wang, X., Dong, G., & Sheng, C. (2020). Discovery of Novel PDEδ Degraders for the Treatment of KRAS Mutant Colorectal Cancer. Journal of Medicinal Chemistry, 63(14), 7892-7905.（IF 7.446）
good7. FU, Leming, et al. Autosomal recessive rod-cone dystrophy associated with compound heterozygous variants in ARL3 gene. Frontiers in cell and developmental biology, 2021, 9: 473.（IF 6.684）
good8. Yu, S., Du, M., Yin, A., Mai, Z., Wang, Y., Zhao, M., ... & Chen, T. (2020). Bcl-xL inhibits PINK1/Parkin-dependent mitophagy by preventing mitochondrial Parkin accumulation. The International Journal of Biochemistry & Cell Biology, 122, 105720. （IF 5.085）
good9. Zhu Y, Tao Z, Chen Y, et al. Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT[J]. Breast Cancer Research and Treatment, 2022: 1-17.（IF 4.872）
good10. Sun Z, Li J, Luo G, et al. Pharmacological activation of SIRT1 by metformin prevented trauma-induced heterotopic ossification through inhibiting macrophage mediated inflammation[J]. European Journal of Pharmacology, 2021, 909: 174386.（IF 4.432）
good11. Cai, Y., Li, X., Pan, Z., Zhu, Y., Tuo, J., Meng, Q., ... & Pan, Y. (2020). Anthocyanin ameliorates hypoxia and ischemia induced inflammation and apoptosis by increasing autophagic flux in SH-SY5Y cells. European Journal of Pharmacology, 883, 173360. （IF 4.432）
good12. Yuan, J., Jiang, Q., Song, L., Liu, Y., Li, M., Lin, Q., ... & Liu, D. (2020). L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction. Molecules, 25(1), 176. （IF 4.411）
good13. ZHANG, Lujia, et al. Autosomal Recessive Retinitis Pigmentosa Associated with Three Novel REEP6 Variants in Chinese Population. Genes, 2021, 12.4: 537.（IF 4.096）
good14. Zhou, S. Z., Zhou, Y. L., Ji, F., Li, H. L., Lv, H., Zhang, Y., & Xu, H. (2018). Analgesic effect of methane rich saline in a rat model of chronic inflammatory pain. Neurochemical research, 43(4), 869-877.（IF 3.996）
good15. Fan, Z., Luo, H., Zhou, J., Wang, F., Zhang, W., Wang, J., ... & Liang, A. (2020). Checkpoint kinase‑1 inhibition and etoposide exhibit a strong synergistic anticancer effect on chronic myeloid leukemia cell line K562 by impairing homologous recombination DNA damage repair. Oncology reports, 44(5), 2152-2164. （IF 3.906）
good16. CHEN, Lixiao, et al. RNA methyltransferase NSUN2 promotes hypopharyngeal squamous cell carcinoma proliferation and migration by enhancing TEAD1 expression in an m5C-dependent manner. Experimental Cell Research, 2021, 112664.（IF 3.905）
good17. Huang, D., Xiong, M., Xu, X., Wu, X., Xu, J., Cai, X., ... & Zhou, H. (2020). Bile acids elevated by high-fat feeding induce endoplasmic reticulum stress in intestinal stem cells and contribute to mucosal barrier damage. Biochemical and Biophysical Research Communications, 529(2), 289-295. （IF 3.575）
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good19. Wang Y, Chen X, Yao N, et al. MiR-448 suppresses cell proliferation and glycolysis of hepatocellular carcinoma through inhibiting IGF-1R expression[J]. Journal of Gastrointestinal Oncology, 2022, 13(1): 355.（IF 2.892）
good20. WU, Hailun, et al. α-Tomatine, a novel early-stage autophagy inhibitor, inhibits autophagy to enhance apoptosis via Beclin-1 in Skov3 cells. Fitoterapia, 2021, 152: 104911.（IF 2.882）