EliKine™ Mouse PDGF-BB ELISA定量试剂盒（一步法）

货号：KTE4053
反应性：小鼠样本专属检测 | 检测方法：双抗体夹心法

核心参数

• 检测范围：25-2000 pg/mL（宽动态线性）
• 超敏阈值：5 pg/mL（同类试剂盒灵敏度领先）
• 样本兼容性：血清/血浆/组织裂解液/细胞上清液

标准化组分包

• 预包被96孔微孔板（含PDGF-BB特异性抗体）
• 梯度标准品（4000 pg/mL冻干粉+专用稀释液）
• 生物素化检测抗体+链霉亲和素-HRP复合物
• TMB显色系统（A/B双组分）

技术优势

▸ 零交叉反应：经严格验证无类似物干扰
▸ 批次稳定性：CV值＜8%（国际ELISA质控标准）
▸ 操作革新：一步法流程，较传统ELISA节省50%操作时间

实验方案

1. 样本预处理：离心去除颗粒物（≥10,000×g，5min）
2. 标准品梯度重建：采用专用稀释液进行8梯度稀释
3. 双孔平行检测：推荐20μL样本体积+30min室温孵育
4. 自动化判读：450nm波长OD值与浓度正相关（r²＞0.99）

⚠ 关键质控点：
• 板条开封后需密封保存（含干燥剂）
• 显色阶段需避光振荡（800rpm，3min）
• 终止反应后30分钟内完成读数

应用场景

肿瘤微环境研究 | 血管生成机制探索 | 创伤修复模型构建
*本产品符合ISO13485质量管理体系，仅供科研使用

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse PDGF-BB ELISA Quantitation Kit (One-Step Procedure)

Cat. No.: KTE4053
Reactivity: Specific for mouse samples | Assay Type: Sandwich ELISA

Key Specifications

• Detection Range: 25-2000 pg/mL (Wide dynamic linearity)
• Ultra-sensitive Threshold: 5 pg/mL (Industry-leading sensitivity)
• Sample Compatibility: Serum/Plasma/Tissue lysate/Cell culture supernatant

Standardized Components

• Pre-coated 96-well Microplate (with PDGF-BB specific antibody)
• Standard Set (4000 pg/mL lyophilized powder + dedicated diluent)
• Biotinylated Detection Antibody + Streptavidin-HRP Conjugate
• TMB Chromogenic System (Component A/B)

Technical Advantages

▸ Zero Cross-Reactivity: Rigorously validated against structural analogs
▸ Batch Consistency: CV <8% (International ELISA QC standards)
▸ Procedural Innovation: 50% time-saving compared to conventional ELISA

Protocol Outline

1. Sample Preparation: Clarify by centrifugation (≥10,000×g, 5min)
2. Standard Reconstitution: 8-point dilution series with dedicated diluent
3. Duplicate Assay: Recommended 20μL sample volume + 30min RT incubation
4. Automated Quantification: 450nm OD value concentration correlation (r²>0.99)

⚠ Critical QC Points:
• Seal unused wells with desiccant immediately
• Light-protected agitation during chromogenic step (800rpm, 3min)
• Complete reading within 30min post-stop solution

Application Fields

Tumor microenvironment research | Angiogenesis mechanism studies | Wound healing model establishment
*Manufactured under ISO13485 Quality Management System. For research use only

Note: Specifications subject to change without prior notice. Contact ACROBiosystems for updated information.