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重组人TNF相关的凋亡诱导配体（TRAIL）
货号：PRP1028 | 产品类型：细胞因子
产品概述
本品为无标签人源重组TRAIL蛋白，基于大肠杆菌表达系统制备，适用于细胞培养及凋亡机制研究。严格遵循质量控制标准，活性经C62H86N12O16协同细胞毒性试验验证，ED50为26.27 ng/mL。
关键参数
- 种属来源：人（UniProt ID：P50591）
- 分子量：19.5 kDa（168个氨基酸）
- 纯度：＞92%（SDS-PAGE检测）
- 内毒素：＜1.0 EU/μg（LAL法检测）
- 表达宿主：大肠杆菌
- 产品形式：冻干粉（20 mM Tris，150 mM NaCl，pH 8.0）
产品特点
- 高选择性凋亡诱导：优先靶向转化及肿瘤细胞，保留正常细胞活性
- 严格质控：批间一致性验证，提供可追溯检测报告
- 即用型包装：含复溶缓冲液建议方案（浓度≥100 µg/mL）
保存与运输
- 长期保存：冻干粉置于＜-20℃干燥环境
- 复溶后保存：4℃稳定2-7天，长期存储需分装于＜-20℃（建议添加0.1% HSA/BSA）
- 运输条件：全程蓝冰冷链运输
规格与价格
| 规格 | 价格（CNY） | 货期 |
|---|---|---|
| 10 μg | 588 | 现货 |
| 50 μg | 1498 | 现货 |
| 100 μg | 1816 | 现货 |
| 1 mg | 请咨询 | |
注意事项
- 使用前需离心处理冻干粉
- 避免反复冻融，推荐工作液分装存储
- 本品仅限研究用途，不适用于临床诊断
背景信息
TRAIL（TNFSF10）属于肿瘤坏死因子超家族，通过激活MAPK8/JNK和Caspase级联反应（Caspase-8/3）选择性诱导肿瘤细胞凋亡（Gene ID：8743）。其作用机制与正常细胞耐受性差异使其成为肿瘤治疗研究的关键靶点。
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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。
Recombinant Human TNF-Related Apoptosis-Inducing Ligand (TRAIL)
Catalog Number: PRP1028 | Product Type: Cytokine
Product Overview
This product is a tag-free human recombinant TRAIL protein produced in an E. coli expression system, suitable for cell culture and apoptosis mechanism studies. Strict quality control standards are followed, with activity validated by actinomycin D synergistic cytotoxicity assay (ED50: 26.27 ng/mL).
Key Parameters
- Species: Human (UniProt ID: P50591)
- Molecular Weight: 19.5 kDa (168 amino acids)
- Purity: >92% (SDS-PAGE analysis)
- Endotoxin: <1.0 EU/μg (LAL assay)
- Expression Host: Escherichia coli
- Formulation: Lyophilized powder (20 mM Tris, 150 mM NaCl, pH 8.0)
Features
- High Apoptosis Selectivity: Preferentially targets transformed and tumor cells while sparing normal cells
- Stringent QC: Batch-to-batch consistency verified with traceable test reports
- Ready-to-Use: Includes recommended reconstitution buffer (concentration ≥100 µg/mL)
Storage & Shipping
- Long-Term Storage: Lyophilized powder at <-20°C in dry conditions
- Post-Reconstitution: Stable at 4°C for 2-7 days; aliquot and store at <-20°C for long-term storage (0.1% HSA/BSA recommended)
- Shipping Conditions: Blue ice cold chain transport
Specifications & Pricing
| Specification | Price (CNY) | Availability |
|---|---|---|
| 10 μg | 588 | In stock |
| 50 μg | 1498 | In stock |
| 100 μg | 1816 | In stock |
| 1 mg | Contact us | |
Notes
- Centrifuge lyophilized powder before use
- Avoid repeated freeze-thaw cycles; aliquot working solutions
- For research use only. Not for clinical diagnosis.
Background
TRAIL (TNFSF10), a member of the tumor necrosis factor superfamily, selectively induces tumor cell apoptosis through activation of MAPK8/JNK and Caspase cascades (Caspase-8/3) (Gene ID: 8743). Its differential mechanism between tumor and normal cells makes it a key target in cancer therapy research.
Note: The above information may be subject to changes due to product optimization. For updated details, please contact Yakoonin Corporation.