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重组人/小鼠/大鼠脑源性神经营养因子（BDNF）
PRP1066 | Human/Mouse/Rat BDNF protein
核心参数：
- 分子量：14.45 kDa（SDS-PAGE验证14 kDa）
- 纯度：＞98%（SDS-PAGE检测）
- 生物活性：ED50＜2 ng/mL（TrkB-BaF3细胞增殖实验）
- 内毒素：＜0.1 EU/μg（LAL法检测）
产品优势
无标签重组蛋白｜大肠杆菌表达系统｜三物种交叉活性｜即用型冻干制剂
技术规格
| 表达宿主 | 大肠杆菌 |
|---|---|
| 蛋白序列 | His129-Arg247（C端6×His标记） |
| 种属来源 | 人/小鼠/大鼠多物种兼容 |
| 溶解建议 | 无菌10 mM HCl（≥100 μg/mL） |
规格与定价
- 5μg ￥588（现货）
- 20μg ￥1,498（现货）
- 100μg ￥3,998（咨询货期）
- 1mg 定制服务（专属报价）
保存与运输：
-20℃冻干保存｜冰袋运输｜复溶后建议分装冻存（含稳定剂体系）
注意事项
- 开盖前3000rpm离心5分钟
- 溶解过程禁止振荡
- 避免反复冻融（建议分装量≤50μL）
应用领域：
神经元培养｜突触可塑性研究｜神经退行性疾病模型构建
*本产品仅供科研使用，不适用于临床诊断
注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。
Recombinant Human/Mouse/Rat Brain-Derived Neurotrophic Factor (BDNF)
PRP1066 | Human/Mouse/Rat BDNF protein
Key Parameters:
- Molecular Weight: 14.45 kDa (14 kDa verified by SDS-PAGE)
- Purity: >98% (determined by SDS-PAGE)
- Biological Activity: ED50 <2 ng/mL (TrkB-BaF3 cell proliferation assay)
- Endotoxin: <0.1 EU/μg (LAL assay)
Product Advantages
Tag-free recombinant protein | E. coli expression system | Cross-species activity | Ready-to-use lyophilized formulation
Technical Specifications
| Expression Host | E. coli |
|---|---|
| Protein Sequence | His129-Arg247 (C-terminal 6×His tag) |
| Species Source | Human/Mouse/Rat multi-species compatible |
| Reconstitution Suggestion | Sterile 10 mM HCl (≥100 μg/mL) |
Specifications & Pricing
- 5μg ￥588 (In stock)
- 20μg ￥1,498 (In stock)
- 100μg ￥3,998 (Lead time inquiry)
- 1mg Custom service (Exclusive quotation)
Storage & Shipping:
Lyophilized at -20℃ | Shipped with ice packs | Recommend aliquoting after reconstitution (contains stabilizer system)
Precautions
- Centrifuge at 3000 rpm for 5 minutes before opening
- Avoid shaking during reconstitution
- Avoid repeated freeze-thaw cycles (recommended aliquot volume ≤50μL)
Application Fields:
Neuronal culture | Synaptic plasticity research | Neurodegenerative disease model construction
*This product is for research use only, not for clinical diagnosis
Note: The above information may change due to product optimization and upgrades. For specific information, please consult Abbkine.