PurKine™ 生物素标签蛋白纯化试剂盒(链霉亲和素)
货号:KTP2030 | 产品分类:核心产品
产品应用:蛋白类(一级应用:细胞器提取和染色)
产品中心:样品制备及检测 | 产品类型:蛋白纯化
产品描述
本试剂盒专为高效纯化生物素化分子(如抗体、蛋白质、核酸及复合体)设计,采用链霉亲和素预装柱技术,提供标准化缓冲液配置,适用于研究级生物分子分离。
特点与优势
- 高载量:每毫升树脂结合≥6 mg生物素化蛋白或>120nmol生物素基团。
- 高性能:低等电点(pI=5)及无糖基化特性显著降低非特异性结合。
- 成本效益:树脂可重复使用>5次,性能无损耗。
- 灵活适配:提供树脂填料、预装柱或完整试剂盒多形态选择。
- 操作便捷:预优化缓冲液体系简化实验流程。
- 机械稳定性:高交联珠粒耐受线性流速高达500 cm/h。
试剂盒组分
- PurKine™ 生物素标签-链霉亲和素预装柱 6FF
- 结合与洗涤缓冲液(10X)
- 洗脱缓冲液(10X)
存储与运输
保存建议:2-8°C保存(自收货日起有效期12个月),禁止冷冻。
运输条件:冰袋(蓝冰)冷链运输。
规格与订购
规格 | 价格(¥) | 货期 |
---|---|---|
1 mL | 1,916 | 期货(5个工作日内发货) |
1 mL×5 | 4,758 | 期货(5个工作日内发货) |
注意:本品仅限研究用途,不适用于人类或诊断应用。使用前请确认符合当地法规及知识产权要求,我方不承担由应用本品引发的任何法律责任。
技术背景
基于生物素-链霉亲和素高亲和力结合系统(Kd≈10^-15M),广泛用于靶向分子定向捕获与纯化。
搜索关键词
生物素标签蛋白纯化 | Streptavidin resin | KTP2030 | Biotin tag resin
```注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。
PurKine™ Biotin-Tagged Protein Purification Kit (Streptavidin)
Catalog No.: KTP2030 | Product Category: Core Products
Applications: Protein-related (Primary Application: Organelle Extraction and Staining)
Product Center: Sample Preparation & Detection | Product Type: Protein Purification
Product Description
This kit is specifically designed for high-efficiency purification of biotinylated molecules (e.g., antibodies, proteins, nucleic acids, and complexes) using streptavidin pre-packed columns. It provides standardized buffer configurations and is suitable for research-grade biomolecular separation.
Features & Advantages
- High Binding Capacity: ≥6 mg biotinylated protein or >120 nmol biotin groups per mL resin.
- High Performance: Low isoelectric point (pI=5) and non-glycosylated properties significantly reduce non-specific binding.
- Cost-Effective: Resin can be reused >5 times without performance degradation.
- Flexible Options: Available in resin, pre-packed columns, or complete kit formats.
- User-Friendly: Pre-optimized buffer system simplifies experimental procedures.
- Mechanical Stability: Highly cross-linked beads withstand linear flow rates up to 500 cm/h.
Kit Components
- PurKine™ Biotin Tag-Streptavidin Pre-packed Column 6FF
- Binding & Wash Buffer (10X)
- Elution Buffer (10X)
Storage & Shipping
Storage: 2-8°C (12-month shelf life upon receipt). Do not freeze.
Shipping: Ice packs (blue ice) for cold chain transportation.
Specifications & Ordering
Specification | Price (¥) | Lead Time |
---|---|---|
1 mL | 1,916 | In stock (shipped within 5 business days) |
1 mL×5 | 4,758 | In stock (shipped within 5 business days) |
Note: This product is for research use only. Not intended for human or diagnostic applications. Ensure compliance with local regulations and intellectual property requirements before use. We assume no liability for any legal consequences arising from the application of this product.
Technical Background
Based on the high-affinity biotin-streptavidin binding system (Kd≈10^-15M), widely used for targeted molecule capture and purification.
Search Keywords
Biotin-tagged protein purification | Streptavidin resin | KTP2030 | Biotin tag resin
Note: The above information may be subject to changes due to product optimization and upgrades. For detailed information, please consult AcroBio.