PurKine™ 组氨酸标签蛋白纯化试剂盒（Ni-NTA树脂）KTP2001

◆ 产品定位与应用场景

核心级蛋白纯化工具，专为组氨酸标签融合蛋白设计，适用于细胞器提取后的靶蛋白捕获及包涵体变性纯化双重场景。兼容天然/变性条件，满足结构生物学及体外功能研究需求。

◆ 技术参数矩阵

• 树脂载量：≥50 mg 6xHis-融合蛋白/mL树脂
• 循环次数：≥5次性能稳定（建议0.5 M NaOH再生）
• 缓冲体系：预配10X PBS + 梯度咪唑溶液

◆ 多形态交付方案

规格	价格（CNY）	库存状态
1 mL	769	期货（2日内交付）
1 mL×5	1916	当日发货（库存≥200套）

◆ 操作体系兼容性

适配IMAC标准工作流程：
镍柱与IDA/NTA配体协同作用，支持：
• 天然条件（pH 7.4-8.5）温和洗脱
• 变性条件（4-8 M尿素/盐酸胍）强力溶解

▣ 冷链管理声明

• 储运温度：2-8°C恒温（避光防冻）
• 有效期：生产批号+12个月
• 运输保障：医用级蓝冰+独立冷链箱

! 重要使用声明

本产品仅限科研用途，不符合GMP生产规范。建议配合His标签验证试剂（Western Blot/HPLC）进行纯度验证，具体应用场景请自行确认专利合规性。

技术备注：基于IMAC原理的镍柱介质，建议结合Co²⁺螯合介质（货号KTP2002系列）进行交叉验证。

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

PurKine™ His-Tagged Protein Purification Kit (Ni-NTA Resin) KTP2001

◆ Product Positioning & Applications

Core-grade protein purification tool specifically designed for His-tagged fusion proteins, suitable for both target protein capture post-organelle extraction and inclusion body denaturing purification. Compatible with native/denaturing conditions, meeting structural biology and in vitro functional study requirements.

◆ Technical Parameter Matrix

• Resin Capacity: ≥50 mg 6xHis-fusion protein/mL resin
• Reusability: ≥5 cycles with stable performance (recommended regeneration with 0.5 M NaOH)
• Buffer System: Pre-formulated 10X PBS + imidazole gradient solutions

◆ Multi-format Delivery Options

Specification	Price (CNY)	Inventory Status
1 mL	769	Futures (delivery within 2 days)
1 mL×5	1916	Same-day shipping (inventory ≥200 sets)

◆ Operational System Compatibility

Compatible with IMAC standard workflows:
Nickel columns synergize with IDA/NTA ligands, supporting:
• Gentle elution under native conditions (pH 7.4-8.5)
• Strong solubilization under denaturing conditions (4-8 M urea/guanidine HCl)

▣ Cold Chain Management Statement

• Storage/Transport Temperature: 2-8°C constant (light-sensitive, freeze-protected)
• Shelf Life: 12 months from production batch
• Transport Assurance: Medical-grade blue ice + independent cold chain box

! Critical Usage Statement

For research use only, non-GMP compliant. Recommended to pair with His-tag validation reagents (Western Blot/HPLC) for purity verification. Users must independently confirm patent compliance for specific applications.

Technical Note: IMAC-based nickel column media should be cross-validated with Co<sup>2+</sup>-chelating media (Catalog No. KTP2002 series).

Note: The above information may be subject to changes due to product optimization. For updated details, please consult Yakoyin Company.