彗星法DNA损伤分析试剂盒（3孔载玻片） | Comet Assay Kit (3-Well Slides) KTA3040

产品定位

▸ 核心产品 | 细胞分析中心 | 细胞凋亡/周期检测 ▸

检测原理

基于单细胞凝胶电泳技术（SCGE），通过PI荧光染色定量分析DNA断裂损伤。正常DNA保留于核区，断裂碎片形成彗星拖尾，损伤程度与拖尾长度/荧光强度呈正相关。

核心组分

• • 彗星专用载玻片（3孔/片）
• • 低熔点琼脂糖（预优化浓度）
• • 10×裂解液 & 500 mM EDTA缓冲液
• • 50×碘化丙啶（PI）染色液

技术优势

• ▶︎ 3孔玻片专利设计：减少样本交叉干扰，支持平行实验对照
• ▶︎ 免铺胶工艺：预置琼脂糖基质层，样本加载效率提升40%
• ▶︎ 超强附着力：通过ISO 13485认证的玻片涂层技术，脱落率＜0.5%

规格与交付

• • 15T | ￥1498 | 现货（24H发货）
• • 75T | ￥4198 | 现货（24H发货）

操作规范

• ⚠️ 禁止混用不同批次组分
• ⚠️ 裂解时间建议控制在1.5-2小时（25℃避光）
• ⚠️ 电泳参数：20V/cm，30分钟（碱性缓冲液）

质控声明

每批次试剂盒通过Hela细胞阳性对照验证，提供DNA损伤梯度标准曲线（随货附电子版）。

重要警示

※ 本产品仅限科研用途，不适用于临床诊断或体内实验 ※
※ 使用前请确认实验方案符合所在地生物安全法规 ※

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Comet Assay DNA Damage Analysis Kit (3-Well Slides) | Comet Assay Kit (3-Well Slides) KTA3040

Product Positioning

▸ Core Product | Cell Analysis Center | Apoptosis/Cell Cycle Detection ▸

Detection Principle

Based on Single Cell Gel Electrophoresis (SCGE), DNA strand breaks are quantitatively analyzed via PI fluorescent staining. Intact DNA remains in the nuclear region, while fragmented DNA forms a comet tail. The extent of damage correlates positively with tail length/fluorescence intensity.

Core Components

• • Comet-specific slides (3 wells/slide)
• • Low-melting-point agarose (pre-optimized concentration)
• • 10× Lysis Buffer & 500 mM EDTA Buffer
• • 50× Propidium Iodide (PI) Staining Solution

Technical Advantages

• ▶︎ Patented 3-Well Slide Design: Minimizes cross-contamination and supports parallel experimental controls
• ▶︎ No-Gel-Coating Process: Pre-coated agarose matrix layer improves sample loading efficiency by 40%
• ▶︎ Superior Adhesion: ISO 13485-certified slide coating technology with <0.5% detachment rate

Specifications & Delivery

• • 15T | ¥1498 | In Stock (Shipped within 24H)
• • 75T | ¥4198 | In Stock (Shipped within 24H)

Operational Guidelines

• ⚠️ Do not mix components from different batches
• ⚠️ Recommended lysis time: 1.5-2 hours (25°C, protected from light)
• ⚠️ Electrophoresis parameters: 20V/cm, 30 minutes (alkaline buffer)

Quality Control Statement

Each batch is validated using Hela cell positive controls and includes a DNA damage gradient standard curve (electronic version provided with shipment).

Important Warning

※ For research use only. Not intended for clinical diagnosis or in vivo experiments ※
※ Ensure compliance with local biosafety regulations before use ※

Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult Yakoyin Company.