纯度≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.内毒素< 1.0 EU per μg protein as determined by the LAL method.生物活性1.Immobilized Recombinant Human IL-31 Protein (His & AVI Tag), Biotinylated (Cat: 11557-H49H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (5 μg/mL, 100 μL/well) can bind Recombinant Human Interleukin-31 receptor A / IL31RA Protein (Fc Tag, ECD) (Cat: 15878-H02H), the EC₅₀ is 0.4-1.2 μg/mL(QC tested).
2.Immobilized Recombinant Cynomolgus IL31 Protein (His Tag) (Cat: 90895-C08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human Interleukin-31 receptor A / IL31RA Protein (Fc Tag, ECD) (Cat: 15878-H02H), the EC₅₀ is 242.5 ng/mL (Routinely tested).
3.Immobilized Recombinant Human IL-31 / IL31 Protein (His Tag) (Cat: 11557-H08H) at 1 μg/mL (100 μL/well) can bind Recombinant Human Interleukin-31 receptor A / IL31RA Protein (Fc Tag, ECD) (Cat: 15878-H02H), the EC₅₀ is 1.27 μg/mL (Routinely tested).蛋白构建A DNA sequence encoding the human IL31RA (EAW54932.1) (Met1-Ser516) was expressed with the Fc region of human IgG1 at the C-terminus.Accession#EAW54932.1表达宿主HEK293 Cells种属Human预测 N 端Ala 20分子量The recombinant human IL31RA consists 735 amino acids and predicts a molecular mass of 83.5 kDa.缓冲液Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.