Biorheological Properties of Reconstructed Erythrocytes and its Function of Carrying-Releasing Oxygen 2009, Vol. 37, No. 1 , Pages 41-44 Xiang Wang1†, Wei Gao1, Weiyan Peng1, Jiaxin Xie1 and Yaojin Li1 1College of Bioengineering, Chongqing University, Chongqing, P.R. CHINA †Correspondence: Xiang Wang, College of Bioengineering, Chongqing University, Chongqing, 400044, P.R. China xwangchn@vip.sina.com Erythrocyte shape and biomechanical properties have close relation to its physiological function. In this research the erythrocyte was reconstructed with natural structure protein and lipids based on cellular mechanics and hemorheology concepts. The biomechanical properties of the reconstructed erythrocyte were determined with the micropipette aspiration system. The shapes of reconstructed erythrocyte were obtained with electron scanning microscope. The oxygen carrying-releasing function was analyzed with the HEMOX analyzer from TCS, the experimental results indicated that the reconstructed erythrocytes were similar to the natural erythrocyte: having biconcave disc shape, good deformability and carrying-releasing oxygen function. TCS Customers: Hemox Analyzers are installed at leading hospitals and research centers worldwide.
Abbott Laboratories (USA) Auckland University (Australia) Baxter Healthcare Corp. (USA) Cambridge University (England) Centers for Disease Control (USA) Children's Hospital of Philadelphia/University of PA (USA) Hospital Maggiore (Italy) INSERM (France) Jordan University Hospital (Jordan) Hospital San Carlos (Spain) Mayo Clinic (USA) National Institutes of Health (USA) Oak Ridge National Laboratory (USA) Osaka Medical School (Japan) Rockefeller University (USA) Somatogen (USA) Tokyo University (Japan) Uniformed Services University of the Health Sciences (USA - Military) University of Geneva (Switzerland) University of Lisbon (Portugal) Upjohn Company (USA) Wellcome Research Laboratories (England) Westmead Hospital/University of Sydney (Australia)
http://www.stanfordlab.com/LabTestGuide/Search.aspx?TestName=O Stanford Hospital Clinical Lab Test Directory
Oxygen Dissociation P50, RBC
Order Code: 11986R
Specimen Type:
Whole blood
Container Type:
Green-top tube (sodium heparin)
Required Volume:
5 mL
Minimum Volume (Pediatric):
1 mL
Methodology:
Hemox-Analyzer (Measures and Plots 02 Saturation
Standard Run Times:
Mon-Sat
Turnaround Time:
3 days
Special Handling:
Specimens must arrive within 72 hours of draw. Draw blood in a green-top (heparin) tube(s), refrigerate specimen immediately after draw, and send 5 mL of fresh heparinized whole blood refrigerated. Do not transfer blood to other containers. Include a control specimen drawn at the same time from a normal, unrelated, non-smoking individual: draw blood in a green-top (heparin) tube(s), refrigerate specimen immediately after draw, and send 5 mL of fresh heparinized whole blood refrigerated. Do not transfer blood to other containers. Label clearly on outermost label normal control. Rubber-band patient specimen and control vial together.