Human TNF-alpha protein
产品名：Human TNF-alpha protein
货号：orb257883
产品中文名：Human TNF-alpha 蛋白

Catalog Number	orb257883
Category	Proteins
Description	Human TNF-alpha, premium grade is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).
Reactivity	Human
Tag	Native
Form/Appearance	Powder
Purity	95%
Conjugation	Unconjugated
MW	17.4 kDa
Target	TNF-alpha
UniProt ID	P01375
Source	Human TNF-alpha, premium grade (orb257883) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).
Expression System	HEK293
Biological Origin	Human
Biological Activity	Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Expression Region	Val 77 - Leu 233
Endotoxins	0.01 EU per ug
NCBI	NP_000585.2
Buffer/Preservatives	PBS, pH7.4
Note	For research use only
Application notes	This protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date	6 months from date of receipt.

 

 

SDS-PAGE analysis of Human TNF-alpha protein


Line graph illustrates about the sensitivity range of Human TNF-alpha protein using different concentrations 

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade (Cat. No. orb257883) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

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