Reporter Cell Lines：Do you have trouble tracking the engrafted cells in vivo? Do you want to monitor gene expression or protein trafficking dynamically? If the answer is yes, the reporter cell line will be an ideal tool for you.

Benefited from the discovery of reporter genes/proteins (e.g., green fluorescent protein (GFP), luciferase), cell lines are engineered to integrate reporter expression cassettes into its genomes. With the ‘visible’ tag, these reporter cell lines are valuable tools for various scientific studies，such as analysis of gene expression and regulation, cell labeling and in vivo imaging, tumor evaluation, and drug screening.

With robust virus production technology as well as years of experience, scientists at Alstem have established a growing library of reporter cell lines to meet your experimental demand. Our current collections include but not limit to luciferase/GFP, luciferase/tdTomato, nanoluciferase/tdTomato dual reporters cell lines. The type of donor cell covers HEK293, HeLa, Jurkat, A375, HCT116, MC38, etc.

Description：Luciferase catalyzes the substrate luciferin in the presence of Mg2+ and ATP, resulting in a flash of light that decays rapidly. This light can be detected by a luminometer. Luciferase from the firefly has become one of the more widely used reporter proteins for both in vitro and in vivo studies, such as, gene transcription activity assay, the noninvasive imaging of reporter gene expression, imaging cerebral strokes, and tracking genetically engineered T cells. Luciferase/tdTomato dual-reporter A375 (A375-Luc-RFP) cell line is derived from A375 cells by transduction of ALSTEM’s pLenti-SFFV-Luciferase-PGK-tdTomator-T2A-PURO Lentiviral Reporter (cat# LV452) lentivirus, which contains Firefly luciferase encoding gene driven by SFFV promoter, followed by tdTomato and puromycin resistance gene by PGK promoter. This cell line stably expresses luciferase at high level. It also expresses a red fluorescent protein (tdTomato) and is resistant to puromycin. This cell line will be a very useful cell line for non-invasive visualization in both in vitro and in vivo experiments.

Cell Line: A375

Reporters: Luciferase, RFP

Selection Marker: Puromycin

Promoter/Transgene: SSFV-Luc-PGK-TdTomato-T2A-puro

Cell Type: Pooled

Figure 1. Panel A: RFP Fluorescence. Dual-labeled A375 cell line expressing tdTomato at 90% cell confluence. The image was taken using a Nikon fluorescent microscope. Panel B: Luciferase activity. Serial dilutions of dual-labeled A375 cells were plated into a 96-well plate. The luciferase activity was tested 6 hrs later. Data are expressed as mean /- SD from duplicates of three independent experiments.   Figure 2. The lentiviral reporter vector contains luciferase gene driven by SFFV promoter, tdTomato and puromycin resistance gene by PGK promoter.

Specifications:

Product Name	Luciferase/tdTomato Dual-Reporter A375 Cell Line
Shipping Condition	Dry Ice - Overnight Shipping
Storage and Stability	Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control	Each vial contains about 1 x 106 cells with >95% viability before freezing. Each lot of cells is tested for luciferase activity, growth and viability following recovery from cryopreservation, and free of mycoplasma and competent lentivirus as well.
Restricted Use	Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.