BioDynami NGS低输入DNA建库试剂盒——illumina和MGI平台

BioDynami开发了高质量的NGS低输入DNA建库试剂盒(illumina和MGI平台)，用于构建低输入DNA量(1 - 400 ng)的高质量NGS文库。该试剂盒允许科学家研究样本有限的DNA，如肿瘤样本、患者样本和其他特别收集的样本(FACS分选等)。该试剂盒的文库转换效率高，输入DNA量低至1 ng。基于BioDynami独特的DNA末端抛光和连接化学，该试剂盒使使库覆盖均匀和低gc偏倚。

The NGS Low Input DNA Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality NGS libraries with low input DNA amount from 1 ng to 400 ng. The kit allows scientist to study samples with limited DNA such as tumor samples, patient samples, and other specially collected samples (FACS sorting etc.). The kit has high library conversion efficiency with as little as 1 ng DNA input. The fast and simple 1.5-hour protocol makes libraries with even coverage and low GC-bias based our unique chemistry for DNA end-polishing and ligation.

该试剂盒需要双链DNA片段(钝的和/或粘的)作为NGS文库制备的输入DNA，并与两种酶促方法产生的DNA片段兼容(如BioDynami DNA片段酶，Cat.# 40061和Cat.# 40062)和机械方法，如超声和雾化等。
The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic approach (for example, BioDynami DNA fragmentation enzymes, Cat.# 40061 and Cat.# 40062) and mechanical approaches such as sonication and nebulization etc.

NGS低输入DNA建库试剂盒工作流程

illumina平台的NGS低输入DNA库准备试剂盒有三种index类型:

Non-index(Cat.# 30022)：库没有index。

Index(Cat.# 30024)：我们的每个引物都包含一个独特的条形码序列，包含6个碱基，可用于识别低输入的DNA文库。库复用多达48个样本是可能的。

Unique dual index(Cat.# 30025)：使用BioDynami的Four-Base Difference Index System，可以使用独特的双索引对多达96个低输入DNA文库进行文库复用。该系统允许我们对8个碱基长度中至少有4个碱基不同的库进行索引。BioDynami独特的双索引引物可以减少测序错误，如解复用错误、扩增错误、读数分配错误、索引交叉污染以及索引跳跃。该试剂盒包括96对预混合的独特索引引物。

MGI平台试剂盒(Cat.# 34024)也适用上述Index。

Three index types are available for the NGS Low Input DNA Library Prep Kit of illumina platform:

Non-index (Cat.# 30022): Libraries do not have index.

Index (Cat.# 30024): Each of our index primers contains a unique barcode sequence with 6 bases that can be used to identify the low input DNA libraries. Library multiplexing up to 48 samples is possible.

Unique dual index (Cat.# 30025): Library multiplexing up to 96 low input DNA libraries is possible with unique dual indexes with our Four-Base Difference Index System. The system allows us to make indexes for the libraries that have at least 4 bases different from each other in the 8-base barcode length. Our unique dual index primers can reduce sequencing errors such as de-multiplexing errors, amplification errors, mis-assignment of reads, index cross-contamination, and also index hopping. The kit  includes 96 pre-mixed unique pairs of index primers.

Indexes are available for the MGI platform kits (Cat.# 34024).

试剂盒优势:

★ 快速的实验方案

★ 过程简单

★ 清理步骤所需的磁珠更少：节省50%以上的成本

★ 低输入DNA量：从1 ng DNA开始

★ 操作时间只有10分钟

★ 总实验时间约1.5小时

★ 即用型主混合物简化了反应设置

★ 反应组分少

Kit advantages:

• • • Fast protocol
      • The hands-on time is only 10 minutes
      • The total protocol time is around 1.5 hours
    • Simple procedure
      • Ready-to-use master mix makes it simple for reaction setup
      • Less reaction components
    • Less magnetic beads required for cleanup steps: Save the cost more than 50%
    • Low input DNA amount: Starts from 1 ng of DNA

比较相同NGS库制备条件下的库转换效率。输入的DNA量分别为1 ng和10 ng。在文库准备前，用Covaris机械剪切DNA。BioDynami试剂盒:NGS低输入DNA文库准备试剂盒(Cat.# 30022)。

Comparison of library conversion efficiency under the same NGS library preparation condition. Input DNA amounts are 1 ng and 10 ng, respectively. DNA was mechanical sheared with Covaris before library prep. BioDynami kit: NGS Low Input DNA Library Prep Kit (Cat. #30022).

比较相同NGS建库条件下的建库率。输入的DNA量为1 ng、10 ng和100 ng。DNA在文库制备前用Covaris机械剪切(Cat. # 30022)。测定PCR周期数。
Comparison of library yield under the same NGS library prep condition. Input DNA amounts are 1 ng, 10 ng, and 100 ng. DNA was mechanical sheared with Covaris before library prep. BioDynami kit (Cat. #30022). PCR cycle numbers were indicated.

产品订购：

产品名称	货号	产品规格
NGS Low Input DNA Library Prep Kit(illumina)	30022S	non-index，24 reactions
NGS Low Input DNA Library Prep Kit(illumina)	30022L	non-index，48 reactions
NGS Low Input DNA Library Prep Kit(illumina)	30024S	index，24 reactions
NGS Low Input DNA Library Prep Kit(illumina)	30024L	index，48 reactions
NGS Low Input DNA Library Prep Kit(illumina)	30025S	Unique dual index，96 reactions
NGS Low Input DNA Library Prep Kit(illumina)	30025L	Unique dual index，192 reactions
NGS Low Input DNA Library Prep Kit(MGI)	34024S	index，24 reactions
NGS Low Input DNA Library Prep Kit(MGI)	34024L	Index，96 reactions