DESCRIPTION

The PAC-1 antibody is derived from the fusion of SP-2 mouse myeloma cells with splenic lymphocytes from BALB/c mice that were immunized with human platelets.

The PAC-1 recognizes an epitope on the glycoprotein IIb/IIIa (gpIIb/IIIa, αIIbβ3) complex of activated platelets at or near the platelet fibrinogen receptor.

FORMAT

Format

FITC

Excitation Source

Blue 488 nm

Excitation Max

494 nm

Emission Max

520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.