Description

HPLC uses new, high efficient particles as the stationary phase, gaining separating capacity. At the same time, liquid chromatographic column has high efficiency and detection sensitivity. Also the flow phase can control and improve the separation process to obtain high selectivity. In addition, due to the use of high-pressure infusion pump, the analysis speed is relatively quicker than classical liquid chromatography. And chiral HPLC is used to lead the enantiomers into chiral environments so that they exhibit physical differences and get separated. It is usually divided into direct and indirect methods. The former is directly separated by chiral mobile phase (CMP) or chiral stationary phase (CSP). The latter uses chiral reagent to carry out precolumn derivatization for the enantiomers, forming some non-enantiomers, which then are separated by conventional stationary phases. For example, we commonly use chiral HPLC to analyze catecholamine substances.