Description

The method for the purification of enantiomers by high performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) is generally referred to as the continuous adsorption and desorption of compounds between the stationary phase (the column) and the mobile phase, so that different compounds can be separated. Because different compounds have different forces between the two (stationary phase and mobile phase). Some chiral substances and spatial isomers have almost the same polarity, so they are difficult to separate. Therefore, special chiral columns are needed to separate them for purification.