本试剂盒采用双抗体夹心ELISA法。用抗小鼠NRG-4抗体包被于酶标板上，实验时样品或标准品中的小鼠NRG-4与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗小鼠NRG-4抗体和辣根过氧化物酶标记的亲和素。抗小鼠NRG-4抗体与结合在包被抗体上的小鼠NRG-4结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，小鼠NRG-4浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中小鼠NRG-4的浓度。

英文名称	Mouse NRG-4 (Neuregulin 4) ELISA Kit
中文名称	小鼠神经调节蛋白4(NRG-4)酶联免疫吸附测定试剂盒
货号	E-EL-M0121c	种属	Mouse/小鼠
规格	96T/Kit (8*12 strips) 48T/Kit (8*6 strips)
检测方法	双抗体夹心法
检测范围	0.313~20ng/mL	灵敏度	0.188ng/mL

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