Biotin-14-dCTP描述

Biotin-14-dCTP是一款已获专利的 dCTP类似物，在嘧啶基的N 4 –位通过一个14-原子连接头连接生物素。这种核苷酸能够在dATP，dGTP，和dTTP存在的条件下通过缺口平移有效地掺入DNA (1)；或者用末端脱氧核苷酸转移酶 (2) 或者随机引物合成 (3) 标记DNA。所提供的材料数量足够标记多达50 µg的 DNA。

性能和质量测试：纯度由反相HPLC评估。

规格

Label or Dye:	Biotin
Product Size:	50 nmol
Concentration:	0.4 mM
Labeling Method:	Direct Labeling
Shipping Condition:	Dry Ice

内容及储存

Biotin-14-dCTP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.

引用及参考文献

Comparison of detection and signal amplification methods for DNA microarrays. Vora GJ, Meador CE, Anderson GP, Taitt CR,
Mol Cell Probes (2008) 22:294-300

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Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase. Flickinger JL, Gebeyehu G, Buchman G, Haces A, Rashtchian A,
Nucleic Acids Res (1992) 20:2382-2382

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Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA. Gebeyehu G, Rao PY, SooChan P, Simms DA, Klevan L
Nucleic Acids Res (1987) 15:4513-4534