Description

CRISPR knockout requires expression of both a single-guide RNA (sgRNA) that targets the specific site for knockout, and expression of the Cas9 nuclease which cuts the DNA at the target site. While these two components can often be expressed from the same plasmid, for many applications, such as CRISPR knockout screens, cells need to be engineered to express the Cas9 nuclease. For this reason, in addition to offering a range of Cas9 expressing plasmids range of Cas9 expressing plasmids, we have a service to make Cas9+ cells for these applications. For our standard service, we transduce with the Cas9+ lenti at several titers, expand and select cells, then confirm Cas9 activity with our CRISPRtest Assay. We provide the culture that has the highest Cas9 activity and healthy cells.

Item	Stable Cas9 Expression Cell Pool Construction (standard design, non-clonal population)
Company	Cellecta
Price	Get QuoteGet Quote
Catalog Number	CSCL-C9-P
Quantity	1 x 10^6 cells
Applications	Make CRISPR Cas9 Cell Line
Sample Type	Lentiviral
Species	Mammalian