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Amersham ECL Full-Range Rainbow Molecular Weight Markers
Full-Range Rainbow Molecular Weight Markers
- Monitor progress of protein electrophoresis and assess transfer efficiency and molecular weight of blotted proteins.without staining
- High intensity, prestained multicolored bands allow distinction of closely related proteins.
- Full-Range Rainbow Markers (Mr 12 000 to 225 000) use ten separate proteins with six different colors.
- High-Range Rainbow Markers (Mr 12 000 to 225 000) use eight separate proteins with six different colors.
- Low-Range Rainbow Markers (Mr 3 500 to 38 000) use seven separate proteins with five different colors.
- For use on SDS-PAGE.
Rainbow Molecular Weight Markers have been enhanced to enable faster and simpler identification of proteins on SDS-polyacrylamide gels. The ready-to-load markers provide sharper, more intense bands on gels and blots and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colors of the markers allow easier confirmation of transfer to blotting membranes and orientation.
Rainbow Molecular Weight Markers comprise a mixture of individually colored proteins that are combined to produce bands of equal color intensity. The approximate protein concentration is 1 to 2 mg/ml, depending on the product. Each 250 µl pack size is sufficient for use with 50 minigels (10 x 8 cm) or 25 large gels (20 x 20 cm).
Amersham ECL Full-Range Rainbow Molecular Weight Markers
| Complete Packsize | 250 µl |
| Order Information | Store at -15°C to -30°C. |
| Application | For industrial applications: Analytical reagent, research Use ten separate proteins with six different colors |
| Number of Tests | Quantities sufficient for use with 50 minigels (10 × 8 cm) or 25 large gels (20 × 20 cm). |
| Molecular Weight [Mr] Min. | Mr 12 000 |
| Molecular Weight [Mr] Max. | Mr 225 000 |
| Concentration | Approx. 2 mg/ml of each protein |
| Kit Contents | 1 vial, 250uL. Supplied ready to use in 305% glycerol and sample buffer containing mercaptoethanesulphonic acid (MESNA) as reducing agent. Sufficient for 25 standard SDS PAGE gel loadings or 50 mini-gel loadings. Full-range rainbow markers (Mr 12 000 to 225 000) use ten separate proteins with six different colors. |
| Physical State | Liquid |
| Color | Brownish-red |
| Solubility | Easily soluble in cold water and hot water |
| Stability | Stable |
| Shelf life | At least 3 months |
| Storage Conditions | -15 to -30°C |
| Wetted Part | No |
| For Use With | SDS-PAGE |