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首页 > Biomedical Research > 人单核细胞趋化蛋白1(MCP-1)ELISA试剂盒
人单核细胞趋化蛋白1(MCP-1)ELISA试剂盒
单价 $388.89对比
询价 暂无
发货 Armenia
品牌 EnkiLife
过期 长期有效
更新 2025-08-09 00:17
 
详细信息

英文名称:MCP-1 (Human) ELISA Kit

指标全称:Monocyte Chemotactic Protein 1

指标别称:CCL2;GDCF-2;HC11;HSMCR30;MCAF;MCP1;SCYA2;SMC-CF;

反应原理(Test Principle):

本试产品采用双抗体夹心ELISA法。用抗Human MCP-1抗体包被于酶标板上,实验时样品(或标准品)中的Human MCP-1会与包被抗体结合。后依次加入生物素化的抗Human MCP-1抗体和HRP酶标记的亲和素,抗Human MCP-1抗体与结合在包被抗体上的Human MCP-1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Human MCP-1浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Human MCP-1的浓度。

 

◤存储(Storage):

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

组份清单(Component List):

Component

Specifications

Human MCP-1 Microplate

96T: 8 wells ×12 strips

48T: 8 wells ×6 strips

Human MCP-1 Standard

96T: 2 vial

48T: 1 vial

Human MCP-1 Biotinylated Detection Ab(100×)

96T:120μL ×1

48T:60μL ×1

HRP Conjugate Concentrate (100×)

96T:120μL ×1

48T:60μL ×1

Standard & Sample Diluent

20mL ×1

Biotinylated Detection Ab Diluent

14 mL ×1

HRP Conjugate Diluent

14 mL ×1

Wash Buffer Concentrate (25×)

30 mL ×1

Substrate Reagent

10 mL ×1

Stop Solution

7 mL ×1