Language
| 产品名称 | Recombinant E.coli Trp B (N-6His) |
| 英文名称 | Tryptophan Synthase β chain/Trp B |
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 | <1 EU/µg as determined by LAL test. |
| 蛋白构建 | Recombinant E.coli Tryptophan Synthase Beta Chain is produced by our E.coli expression system and the target gene encoding Thr2-Ile397 is expressed with a 6His tag at the N-terminus. |
| Accession | P0A879 |
| 表达宿主 | E.coli |
| 种属 | E.coli |
| 预测分子量 | 43.8 KDa |
| 制剂 | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 0.05% Tween 80, pH 8.5. |
| 运输方式 | The product is shipped on dry ice/polar packs.Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 | Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 复溶 |
| 分子别名 |
| Tryptophan synthase beta chain; trpB |
| 背景介绍 |
| Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation. |
注意事项
本司产品仅用于科研，不用于临床诊断和治疗