Language
| 产品名称 | Recombinant E.coli Trp synthase (N-6His) |
| 英文名称 | Tryptophan Synthase |
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 | <1 EU/µg as determined by LAL test. |
| 蛋白构建 | Recombinant E.coli Tryptophan Synthase is produced by our E.coli expression system and the target gene encoding Met1-Ser268&Thr2-Ile397 is expressed with a 6His tag at the N-terminus. |
| Accession | P0A877&P0A879 |
| 表达宿主 | E.coli |
| 种属 | E.coli |
| 预测分子量 | 28.7&43.8 KDa |
| 制剂 | Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. |
| 运输方式 | The product is shipped on dry ice/polar packs.Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 | Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 复溶 |
| 分子别名 |
| Tryptophan synthetase; Tryptophan synthase |
| 背景介绍 |
| Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3′-phosphate (IGP) to give indole and D-glyceraldehyde 3′-phosphate (G3P). Indole is then transferred through a 25-Å hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5-phosphate and catalyzes several pyridoxal 5-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target. |
注意事项
本司产品仅用于科研，不用于临床诊断和治疗