产品信息

别名	Interleukin-31; IL-31; IL31
物种	Human
表达宿主	E.coli
序列信息	Ser24-Thr164
检索号	Q6EBC2
分子量	15.8 kDa
表观分子量	15 kDa

 

产品特性

纯度	>95% as determined by reducing SDS-PAGE.
内毒素	<1.0 EU per µg as determined by LAL test.
保存	Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months.
运输	Ambient temperature or ice pack.
制剂	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复融	Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

背景介绍

Human Interleukin 31 (IL-31) is a Cytokine containing a four-helix bundle structure. It shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. Human IL-31 cDNA encodes a 164 amino acid precursor that contains a 23 amino acid signal peptide and a 141 amino acid mature Protein. Human and mouse IL-31 share 24% sequence identity in the mature region. IL-31 is mainly associated with activated T cells and is preferentially expressed by type 2 helper T cells (Th2). IL-31 signals via a heterodimeric Receptor complex composed of a gp130 related molecule termed IL-31RA (also GPL and GLMR) and an Oncostatin M Receptor (OSM Rβ). The IL-31 Receptor is constitutively expressed by keratinocytes and upregulated by IFNγ on monocytes. GPL/ OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Studies have shown that IL31 induces severe pruritis (itching) and dermatitis in transgenic mice.