货号：  K0491

 相关应用： 

 

DNA 纯化

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	货号	单位规格	单价 (CNY)	数量
	K0491	10 preps	1,572.00
	K0492	25 preps	3,512.00

 

规格

High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Sample Type (General):	Bacteria
Downstream Application:	Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Transfection, Transformation
Product Line:	GeneJET
Product Size:	10 preps

 

描述

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 10 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

• Efficient—high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
• High concentration of DNA purified—> 400 ng/ µL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• Transfection of robust cell lines
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
For Research Use Only. Not for use in diagnostic procedures.

图表

High yields of plasmid purified with GeneJET Maxiprep Kit
Plasmid DNA was purified from E. coli cells using the GeneJET Plasmid Maxiprep Kit and the kits of various vendors using recommended culture volumes and purification protocols." href="https://medicineplatform.com/file/upload/202508/12/152631851.jpg">

High yields of plasmid purified with GeneJET Maxiprep Kit
Plasmid DNA was purified from E. coli cells using the GeneJET Plasmid Maxiprep Kit and the kits of various vendors using recommended culture volumes and purification protocols." href="https://medicineplatform.com/file/upload/202508/12/152631851.jpg">High yields of plasmid purified with GeneJET Maxiprep Kit

Efficient transfection of robust cell lines with plasmid DNA purified using GeneJET Maxiprep Kit

• Plasmid DNA, purified from E. coli cells using the GeneJET Plasmid Maxiprep Kit, gives transfection efficiencies comparable to chromatographically purified, endotoxin-free plasmid. One μg of the high-copy plasmid transfected into various cell lines using TurboFect in vitro transfection reagent.
• CHR: endotoxin-free plasmid.
• GeneJET: plasmid purified using the GeneJET Plasmid Maxiprep Kit.

" href="https://medicineplatform.com/file/upload/202508/12/152631501.jpg">

Efficient transfection of robust cell lines with plasmid DNA purified using GeneJET Maxiprep Kit

• Plasmid DNA, purified from E. coli cells using the GeneJET Plasmid Maxiprep Kit, gives transfection efficiencies comparable to chromatographically purified, endotoxin-free plasmid. One μg of the high-copy plasmid transfected into various cell lines using TurboFect in vitro transfection reagent.
• CHR: endotoxin-free plasmid.
• GeneJET: plasmid purified using the GeneJET Plasmid Maxiprep Kit.

" href="https://medicineplatform.com/file/upload/202508/12/152631501.jpg">Efficient transfection of robust cell lines with plasmid DNA purified using GeneJET Maxiprep Kit