Strep-Tactin^®XT 4Flow^® high capacity columns contain a 4% agarose coupled with the streptavidin variant Strep-Tactin^®XT and are applicable for purification of Strep-tag^®II or Twin-Strep-tag^® fusion proteins via gravity flow. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In comparison to Strep-Tactin^®XT 4Flow^®, Strep-Tactin^®XT 4Flow^® high capacity has a higher density of immobilized Strep-Tactin^®XT, which leads to a 2-fold higher yield of purified protein. 
To reduce the waiting period during the sample and buffer application, the use of the application aid WET FRED is recommended.

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