Minute^TM 无表面活性剂的动物细胞/组织总蛋白提取试剂盒

目录号：SN-006

描述  

无表面活性剂的动物细胞/组织总蛋白提取试剂盒，由优化细胞裂解液和0.6 ml离心管柱组成，能够迅速从动物细胞（昆虫/哺乳动物等）和组织（无脊椎动物和脊椎动物）中提取无表面活性剂的总蛋白。试剂盒中的裂解液不含任何表面活性剂和EDTA。离心管柱技术提取蛋白质，样品量最小可处理20ul，最大可达500ul。从细胞/组织中提取总蛋白质操作时间小于5分钟，得率可达1-5mg/ml。

应用  

提取的蛋白质样品可以应用于蛋白质组学（液相色谱/质谱），IP，ELISA，2D分析，等电聚焦，SDS-PAGE, immunoblottings等。

Figure 1. Comparison of protein profiles of different animal tissues extracted by denaturing lysis buffer and detergent-free extraction buffer. Lane 1, Drosophila cultured cells (SL2); Lane 2, Drosophila adult flies; Lane 3, gold fish muscle; Lane 4, mouse liver. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.

操作步骤  

SN-006

操作流程图

Protocol    

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产品特点  

速度快，操作简单

小样本量：最小可处理20ul

无蛋白丢失，高得率

无需超声处理，煮沸，或反复冻融

不含任何表面活性剂和EDTA

参考文献  

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4. Xu, Wenteng, et al. (2016).Ubiquitin ligase gene neurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis protein ubiquitination. Gene (2016), DOI: 10.1016/j.gene.2016.07.062

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6.Pietras, P., Leśniczak-Staszak, M., Kasprzak, A., Andrzejewska, M., Jopek, K., Sowiński, M., ... & Szaflarski, W. (2021). MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells. International Journal of Molecular Sciences, 22(22), 12121.

7.Minerva, D., Othman, N. L., Nakazawa, T., Ito, Y., Yoshida, M., Goto, A., & Suzuki, T. (2022). A New Chemotactic Mechanism Governs Long-Range Angiogenesis Induced by Patching an Arterial Graft into a Vein. International Journal of Molecular Sciences, 23(19), 11208.

8.Tatsuya Yoshida; Satoya Yoshida; Kohei Inukai; Katsuhiro Kato; Yoshimitsu Yura; et al(2024). ALPK2 prevents cardiac diastolic dysfunction in heart failure with preserved ejection fraction. The FASEB Journal.DOI 10.1096/fj.202402103r