Language
说明：从植物中分离微粒体膜是实验室中常见实验，植物细胞中微粒体部分是植物研究项目的重点。微粒体中富含细胞膜，内质网，高尔基体，液泡膜和其他膜结构。传统的分离方法需要大量的样品量，通过超速离心来分离不同的膜结构，步骤复杂。MM-018试剂盒提供了一种快速，简单无需特殊仪器的分离方法，很小的样品量（200mg）即可提取。优化的缓冲液系统，通过简单的离心可分离水溶性胞浆蛋白和非水溶性微粒体，特别是细胞膜部分。操作时间<1小时，蛋白质得率可达100-200ug/样品
特点：
- 简单，快速，性价比高
- 得率高
- 小样品量
- 无需其他仪器
- 储存条件：储存于-20℃
参考文献：
1.Soohong Kim etal (2018). Endoplasmic reticulum stress‐induced accumulation of VAMP721/722 requires CALRETICULIN 1 and CALRETICULIN 2 in Arabidopsis. Journal of Integrative Plant Biology. doi.org/10.1111/jipb.12728.
2.Zhili Yang et al(2020).PROTEIN PHOSPOHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice.Plant Cell.
3.Yu, F., Cao, X., Liu, G., Wang, Q., Xia, R., Zhang, X., & Xie, Q. (2020). ESCRT-I Component VPS23A Is Targeted by E3 Ubiquitin Ligase XBAT35 for Proteasome-Mediated Degradation in Modulating ABA Signaling. Molecular Plant, 13(11), 1556-1569.
4.Kato, T., Morita, R., Ootsuka, S., Wakabayashi, Y., Aoki, N., & Horibata, A. Evaluation of alleles at OsAGPS2, OsAGPL2, and OsSUT1 related to grain filling in rice in a common genetic background. Crop Science.
5.Hou, L.-Y.; Lehmann, M.;Geigenberger, P. Thioredoxin h2 and o1 Show Different Subcellular Localizations and Redox-Active
Functions, and Are Extrachloroplastic Factors Influencing Photosynthetic Performance in Fluctuating Light.Antioxidants 2021, 10, 705.