产品信息

名称	NCI-H727 (人肺癌细胞) (STR鉴定正确)
别称	H727; H-727; NCIH727
种属	人
生长特性	贴壁细胞
细胞形态	上皮细胞样
冻存条件	冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮
培养方案A（默认）	生长培养基: RPMI-1640＋10% FBS＋1% P/S 培养条件: 气相：空气，95%；CO2，5%, 温度：37℃
推荐传代比例	1:3-1:5
推荐换液频率	3-4次/周

参考资料(来源文献)

背景描述	NCI-H727 [H727] is a cell line exhibiting epithelial morphology that was isolated from the lungs of a 65-year-old, White female with a carcinoid tumor.This is the best differentiated of the available bronchial carcinoid lines. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. The cells are able to synthesize the peptide NMB (at 0.1 pmol/mg protein), but not the gastrin releasing peptide (GRP). The cell line secretes a parathyroid hormone-like protein which is calcium stimulated through a protein kinase C pathway. Growth of NCI-H727 cells is inhibited by epidermal growth factor (EGF) receptor monoclonal antibodies.
年龄（性别）	女性；65岁
组织来源	肺；支气管
细胞类型	肿瘤细胞
肿瘤类型	肺癌细胞
生物安全等级	BSL-1
倍增时间	41 hours (PubMed=29681454)
抗原表达情况	Epidermal growth factor (EGF)
基因表达情况	neuromedin B (NMB)
保藏机构	ATCC; CRL-5815 ECACC; 94060303

STR鉴定图

• STR位点信息

  Amelogenin	X
  CSF1PO	11,12
  D2S1338	17,24
  D3S1358	16
  D5S818	11,12
  D7S820	8,10
  D8S1179	10,14
  D13S317	11
  D16S539	11,13
  D18S51	16,22
  D19S433	13,14

  D21S11	29,32.2
  FGA	20,22
  PentaD	9,14
  PentaE	14
  TH01	8
  TPOX	8
  vWA	14,15
  D6S1043	12,17
  D12S391	20,24
  D2S441	10,11

• STR鉴定图