产品信息

别名	IGIF, IL-1g, IL1F4
物种	Human
表达宿主	E.coli
序列信息	MYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMN,P.PDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED with polyhistidine tag at the C-terminus.
检索号	Q14116.1
分子量	18.2 kDa
表观分子量	15 kDa
标签	His-tag at the C-terminus
生物活性	Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is <5 ng/mL.

产品特性

纯度	>98% as determined by SDS-PAGE. Ni-NTA chromatography
内毒素	<0.1 EU per 1 μg of the protein by the LAL method.
保存	Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution,store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months.
运输	Ambient temperature or ice pack.
制剂	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
复融	It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

背景介绍

Interleukin-18 (L-18) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL-4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.