Unit
5 x 48 Measuring Points: 1 Sample per Strain if tested in Triplicates, 6 Dilutions, +/- 1254 Aroclor-induced S9, Negative and Positive Controls
Description

Ames MPF Kit is a liquid microplate fluctuation assay kit, standardized and quality controlled. It is a ready to use Kit for the Ames mutagenicity assay and includes 10 vials each of semisolid TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA, E.coli WP2 pKM101, Booster, Growth -, Exposure -, Reversion Indicator Media, Positive Controls, Post-Mitochondrial Supernatant S9 from lyophilized Aroclor 1254 induced rat liver. The assay is using the same strains, positives controls and rat liver S9, cofactors as listed in OECD 471 and is quality controlled according to OECD 471. The liquid media used in this assay allow to test successfully pharmaceuticals, (agro-)chemicals, cosmetics, water, soil, many publication are referenced on this homepage. Especially volatile compounds, or genotoxic impurities available in low quantities, e.g. nitrosamines can be tested successfully with the Ames MPF. 

Characteristics

• Quality controlled kit including all strains, S9, media, positive controls - no inhouse quality control, no sterility control are necessary   
• Certificate of Analysis is provided
• Excel Calculation Sheet to support data Interpretation free of charge
• Compliant with ICH M7 allowing testing for very small quantities of genotoxic impurities, e.g. nitrosamines
• Suitable for volatile test compounds
• Number of tests corresponds to 5 x 480 agar plates.
• Standardized procedure
• Shipped at room temperature from external storage in Germany or from Switzerland
• High sensitivity and excellent correlation with agar plate test (see referenced publications)
• 4 times less test compound as compared to the agar plate test, optimized for screening purposes
• Less hands-on-time and simultaneous processing of several replicates
• Automation available - high throughput
• 3Rs - 12 fold less consumption of rat liver liver S9, significant less test animals sacrificed
• Less error prone due to easy colorimetric read-out
• Waste - Significant less plastic waste and thus reduced contaminated waste in Environment

The Ames test is a bacterial mutation reversion assay which evaluates the mutagenic potential of chemical, pharmaceutical or cosmetic substances, food additives, food packaging material, mixtures, drinking-, waste water or soils. The bacterial strains carry several mutations that inactivate the gene involved in the synthesis of histidine (s.typhimurium) or tryptophan (E.coli), i.e. they grow only in a medium supplemented with either his or trp. Mutagens induce mutations which may restore the ability of the bacteria to re-synthesize the amino acid (his or trp) and to grow in medium without his or trp. Growth of bacteria induces a pH shift which is easily detected by the yellow color due to the pH-indicator included in the medium.