Language
- Effective and robust delivery into the cytoplasm 有效和稳健的传递到细胞质
- Delivery to a wide variety of cells including primary cells and Hard-to-transfect cells 可传递到多种细胞，包括原代细胞和难以转染的细胞
- Highly efficient delivery 高效的交付
- Easy to use 易于使用的
Overview（概述）
Specifications（规范）
Reagent
试剂PULSin®
Molecule delivered
转染核酸Protein; Antibody; Peptide
蛋白质;抗体;肽Cell types
细胞类型Adherent & suspension cells grown in presence of serum
粘附细胞和悬浮细胞在血清存在下生长Number of transfection
试剂用量0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates
0.4 ml的PULSin®给药试剂足以在6孔板中进行25个给药实验，在24孔板中进行100个给药实验Storage
储存条件2-8°C, stable for at least one year when stored appropriately
2-8°C，适当储存可稳定保存至少一年Provided with
配套提供Hepes Buffer (20mM) for protein dilution; R-Phycoerythrin used as a positive control
Hepes Buffer (20mM)用于蛋白质稀释;r -藻qing菊酯用作阳性对照
Summary（总结）The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. For example, PULSin® enables you to study lethal proteins by controlling the level and time course of protein delivery into the cells. Similarly, delivery of blocking antibodies may provide additional information to traditional RNA interference experiments*. With PULSin® you can target intracellular proteins with antibodies in living cells without fixation step.
* G. Cassinelli et al. (2006). Inhibition of c-Met and prevention of spontaneous metastatic spreading by the 2-indolinone RPI-1, Mol Cancer Ther 5:9, 2388-2397.
使用PULSin®传递蛋白质和抗体是功能研究的一种强大方法。例如，PULSin®可以通过控制蛋白质进入细胞的水平和时间过程来研究致命蛋白质。同样，阻断抗体的传递可以为传统的RNA干扰实验提供额外的信息*。使用PULSin®，您可以靶向活细胞中的细胞内蛋白，无需固定步骤。
* G. Cassinelli等人(2006)。2-吲哚酮RPI-1抑制c-Met和预防自发转移扩散，Mol Cancer Ther:9, 2388-2397。Ordering information（订购信息）
Reference number Amount of reagent Positive control (R-phycoerythrin) Hepes dilution buffer 101000010 PULSin® 0.4 mL 20 µg 20 ml
PULSin®蛋白、抗体、多肽转染试剂