PCR-based detection of mycoplasma contamination of cell culture.

Mycoplasma is a common and serious contaminant of cell cultures. It has been shown that more then 30% of cell cultures in the laboratory are infected with Mycoplasma. In continuous cell cultures, contaminating Myboplasma may grow slowly without killing the cells but affecting various parameters including altered cellular proliferation and biabiligy, morphological changes, cell transformation, mimicking virus infection, and inresponsiveness to drug treatment, etc., and ultimatedly leading to unreliable results. Mycoplasma detection is an important and necessary quality control measure.

Many of the testing procedures have been developed, which include NNA staining , enzyme-linked immunosorbent assay (ELISA), ploymerase chain reaction (PCR), and PCR-based ELISA. This kit provides our research community with reliable reagents and simple protocol, which allow for rapid and highly reproducible detection of mycoplasma contamination.

The primers used in this kit anneal to conserved regions of the Mycoplasma genome, allowing detection of the most common species of Mcoplasma (including M. Poalescens, M. Arginini; M. Fermentans; M. Caviae, M. Huorhinis, M. Indiense , M. Orale,  Acholeplasma laidlawii and many more).