Millipore货号M8823-1ml抗-FLAG M2磁珠13611631389上海睿安生物

说明

一般描述:抗-FLAG M2磁珠是可与抗-FLAG M2(小鼠单克隆)抗体结合的4％琼脂糖微珠。 该M2抗体可识别FLAG序列的N-末端、Met N-末端和C-末端。 这可实现对含有FLAG肽序列的融合蛋白的检测和捕获。

应用

适用免疫沉淀分析。

洗脱方法:FLAG肽、甘氨酸(pH 3.5)、3x FLAG肽

更多产品信息请访问我们的FLAG应用页面。

特点和优势

该磁性属性可实现：
- 极为快速的分离
- 显著被加速的操作，例如重复的洗涤
-以板式进行多个样本的处理
该可导致：
- 更为快速的实验
- 更好的可重复性
- 目标蛋白更为准确的定量

外形:以含10mM磷酸钠、150mM 氯化钠(pH 7.4)、0.02%(w/v)Die氮化钠(PBA/A)的50%甘油的50%悬浮液供应

 

同行评审论文

Knockdown of lncRNA LEF1-AS1 inhibited the progression of oral squamous cell carcinoma (OSCC) via Hippo signaling pathway.Chanqiong Zhang et al.Cancer biology & therapy, 20(9), 1213-1222 (2019-04-16)It is verified that long non-coding RNAs (lncRNAs) play crucial roles in various cancers. LncRNA LEF1-AS1 is a reported oncogene in colorectal cancer and glioblastoma. In this study, we unveiled that LEF1-AS1 markedly increased in oral squamous cell carcinoma (OSCC)Enteropathogenic Escherichia coli remodels host endosomes to promote endocytic turnover and breakdown of surface polarity.Ephrem G Kassa et al.PLoS pathogens, 15(6), e1007851-e1007851 (2019-06-27)Enteropathogenic E. coli (EPEC) is an extracellular diarrheagenic human pathogen which infects the a_pical plasma membrane of the small intestinal enterocytes. EPEC utilizes a type III secretion system to translocate bacterial effector proteins into its epithelial hosts. This activity, whichA novel signalling screen demonstrates that CALR mutations activate essential MAPK signalling and facilitate megakaryocyte differentiation.K Kollmann et al.Leukemia, 31(4), 934-944 (2016-10-16)Most myeloproliferative neoplasm (MPN) patients lacking JAK2 mutations harbour somatic CALR mutations that are thought to activate cytokine signalling although the mechanism is unclear. To identify kinases important for survival of CALR-mutant cells, we developed a novel strategy (KISMET) thatUBXN3B positively regulates STING-mediated antiviral immune responses.Long Yang et al.Nature communications, 9(1), 2329-2329 (2018-06-15)The ubiquitin regulatory X domain-containing proteins (UBXNs) are likely involved in diverse biological processes. Their physiological functions, however, remain largely unknown. Here we present physiological evidence that UBXN3B positively regulates stimulator-of-interferon genes (STING) signaling. We employ a tamoxifen-inducible Cre-LoxP approachTaiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration.Chao Wang et al.Cell discovery, 2, 16006-16006 (2016-07-28)The Hippo signaling pathway regulates tissue growth and organ size through controlling cell growth, proliferation and apoptosis. During these processes, the coactivator Yorkie partners with the transcription factor Scalloped to mediate Hippo pathway-regulated cellular functions. Here, we demonstrate that Taiman