Sigma货号NUC101-1KT细胞核分离试剂盒(Nuclei EZ Prep)上海睿安生物13611631389

细胞核分离试剂盒:Nuclei EZ Prep

sufficient for 25 nuclei preparations(~1-10×10⁷ cells/preparation)

别名:Rapid mammalian nuclei isolation kit

说明

一般描述:Nuclei EZ Prep Kit用于从哺乳动物细胞中快速分离胞核。该试剂盒可从通用细胞类型获得高得率细胞核。

应用:适于作为细胞核成分的来源，为体外凋亡检测和功能研究提供细胞核成分。

生化/生理作用

配套实验方案使您可从常用的哺乳动物细胞获得高得率的细胞核，包括贴壁(例如，HEK293和COS7)和非贴壁(例如，Jurkat和HFN7.1)细胞系和外周血单核细胞(PBMC)。该制备适用于许多细胞生物学应用，例如:为染色质、基因组DNA、组蛋白和核RNA/RNP提供细胞核来源，为体外凋亡检测、功能性研究(如细胞转录状态检查)制备细胞核。

其他说明:所有操作步骤应在冰块上或2-8°C条件下进行。

同行评审论文(部分文献)

Mutually suppressive roles of KMT2A and KDM5C in behaviour, neuronal structure, and histone H3K4 methylation.Christina N Vallianatos et al.Communications biology, 3(1), 278-278 (2020-06-03)Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me enzymes are associated with neurodevelopmental disorders to date, indicating their important roles in the brain. However, interplay among H3K4me enzymes duringAnalysis of nuclear RNA, Chapter 14.Robert E. Farrell, Jr., ed.RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)Suppression of SRCAP chromatin remodelling complex and restriction of lymphoid lineage commitment by Pcid2.Buqing Ye et al.Nature communications, 8(1), 1518-1518 (2017-11-16)Lymphoid lineage commitment is an important process in haematopoiesis, which forms the immune system to protect the host from pathogen invasion. However, how multipotent progenitors (MPP) switch into common lymphoid progenitors (CLP) or common myeloid progenitors (CMP) during this processHominin-specific regulatory elements selectively emerged in oligodendrocytes and are disrupted in autism patients.Bas Castelijns et al.Nature communications, 11(1), 301-301 (2020-01-18)Speciation is associated with substantial rewiring of the regulatory circuitry underlying the expression of genes. Determining which changes are relevant and underlie the emergence of the human brain or its unique susceptibility to neural disease has been challenging. Here weMassively parallel single-nucleus RNA-seq with DroNc-seq.Naomi Habib et al.Nature methods, 14(10), 955-958 (2017-08-29)Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human一、╳※试剂※╳PBMC分离服务:PBMC/人外周血单个核细胞/PBMC细胞/人外周血单个核细胞/冻存PBMC/hPBMC单个核细胞二、╳※试剂※╳:常规血清优势供应:VivaCell货号C04001-500血清、Access货号51HI血清、Gemini货号900-108血清、Gemini血清、Gibco血清(10099-141澳洲血清、10270-106南美血清、16000-044血清)、BI血清BI货号04-001-1ACS、Sigma货号F8318-500ml澳洲胎牛血清、Sigma货号F8687澳洲胎牛血清、Sigma货号F0193南美胎牛血清[(乌拉圭)胎牛血清]、Sigma货号F2442-500ml特级胎牛血清、ScienCell货号0500澳洲胎牛血清、Cegrogen货号A0500-3010胎牛血清、Cegrogen货号B0500-5010新生牛血清、康源血清血替/血小板裂解液、培养基(包括无血清)、冻存液(包括无血清)，包括:友康YoconMerck(Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)正式授权代理、博奥龙Biodragon独dú家总代理、EFL独dú家总代理、碧云天Beyotime代理、Proteintech、Active Motif正式授权代理☆☆☆☆☆☆Beckman货号A63881纯化磁珠Agencourt AMPure XP(60ml/瓶)/A63882(450ml/瓶)；Beckman货号B23317核酸片段筛选试剂盒Agencourt SPRIselect(5ml/瓶)分子类:Biotium货号41003的GelRed染料、货号31000的EvaGreen染料、Zymo Research、Qiagen饲料类:Research Diets、Dyets三、╳※耗材※╳:    洁特JET耗材(各类常规用实验耗材)、BBSP耗材、赛普Cellpro耗材/KirGen耗材、博麦迪(常规的)各类试剂瓶    Bioplastics    Thermo Fisher Scientific的很多产品线，包括旗下的:    一、Nunc耗材    二、QSP耗材(各类吸头/滤芯吸头、离心管、PCR管等)    三、Nalgene耗材仪器    四、FisherBrand耗材仪器(含各类盖玻片载玻片等)    五、Lab_Serv耗材仪器    六、Finnpipette移液器(如：F1系列、F2系列、F3系列等等各类型号的移液器)    七、Abgene耗材畅销款:我们是常备有大量现货的※╳√√✓✔√✓✔√✓✔上海睿安生物科技有限公司成立于2009年，专注于为生命科学和生物技术领域的客户提供优质的产品与服务。代理了多家欧美知名品牌，如美国Thermo Fisher耗材(包括旗下Nunc、QSP、Nalgene、FisherBrand、Lab_Serv耗材仪器、Finnpipette移液器、Abgene耗材，等等)、默克Merck-Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)、Zymo Research、洁特JET耗材(各类常规用实验耗材)、BBSP耗材、赛普Cellpro耗材、博奥龙Biodragon独dú家总代理、EFL独dú家总代理、碧云天Beyotime代理、Eppendorf、友康Yocon、Research Diets高脂饲料，IBL、BioVendor、Mercodia、ShiBayaji代谢类产品、Axygen-Corning长期大量耗材备货、BioCoat畅销款长期备货，VivaCell血清、Access货号51HI血清、Gemini血清、Gibco血清，BI血清现货、ScienCell血清、Sigma血清、Moregate血清、Cegrogen血清、Pierce发光试剂、Fermentas、Invitrogen、Proteintech、Active Motif、Abcam、CST、BD、SAB、R&D systems、Beckman、Qiagen、BD Pharmingen、Biolegend、Jackson、Miltenyi、MP Biomedicals、Biotium更好的荧光试剂产品等品牌。睿安生物始终将“提供更好的产品和服务”作为企业发展的目标和努力的方向，并将通过优质的售前与售后服务，始终如一地为客户提供高质量的产品和服务，我们也希望能得到相关领域中更多老师和同仁们的指导和鞭策，为共创优秀品牌而努力奋斗！询订※╳直线13611631389微信号