Zymo Research货号D5460现货Zymo-Seq RRBS Library Kit

上海睿安生物13611631389

HIGHLIGHTS

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• Simple workflow: Prepare Reduced Representation Bisulfite Sequencing(RRBS) libraries in as little as 2 hours of hands-on time.
• Low input: The only RRBS kit that produces NGS libraries from ≥10 ng of genomic DNA.
• Accurate and reproducible: Unbiased methylation calling and reproducible CpG coverage.

DESCRIPTION

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Zymo-Seq RRBS Library Kit is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS). This all-in-one RRBS library prep kit allows for DNA methylation profiling at single-nucleotide resolution in CpG-rich regions of the genome. This Zymo-Seq RRBS Library Kit is the ideal RRBS library prep kit to generate libraries that cover a significant percent (≥70% if using human genomic DNA) of all CpG islands, promotors, and gene bodies while largely reduces the sequencing cost, making it ideal for high-throughput screening and comparison experiments.

 

Mixtures of per- and polyfluoroalkyl substances (PFAS) alter sperm methylation and long-term reprogramming of offspring liver and fat transcriptome. DruAnne L Maxwell, Oladele A Oluwayiose, ..., J Richard Pilsner Environment international  |  2024 Mar 16  |  PubMed ID: 38521043  |  Read Article  

".. was performed using the EpiTect Fast DNA bisulfite kit from Qiagen (Cat. # 59824).. RRBS libraries were constructed from Zymo-Seq RRBS library kit according to the manufacture’s guidelines.. Libraries were sequenced on an Illumina NovaSeq in the genomics core facility at Wayne .." More... | Share Article

Genome-wide 5'-C-phosphate-G-3' methylation patterns reveal the effect of heat stress on the altered semen quality in Bubalus bubalis. Gautam Kumar, Ankita Gurao, ..., Ranjit Singh Kataria Gene  |  2024 Mar 05  |  PubMed ID: 38331117  |  Read Article   "Abstract: Semen production and quality are closely correlated with different environmental factors in bovines, particularly for the buffalo (Bubalus bubalis) bulls reared under tropical and sub-tropical conditions. Factors including DNA methylation patterns, an intricate process in sperm cells, have an impact on the production of quality semen in buffalo bulls under abiotic stress conditions. The present study was conducted to identify DNA methylome signatures for semen quality in Murrah buffalo bulls, acclaimed as a major dairy breed globally, under summer heat stress" More... | Share Article  Cellular and molecular alterations in a human hepatocellular in vitro model of nonalcoholic fatty liver disease development and stratification. Rose A Willett, Volodymyr P Tryndyak, ..., Igor P Pogribny Journal of environmental science and heal…  |  2024 Jan 22  |  PubMed ID: 38105681  |  Read Article   "Abstract: The rapidly increasing incidence of nonalcoholic fatty liver disease (NAFLD) is a growing health crisis worldwide. If not detected early, NAFLD progression can lead to irreversible pathological states, including liver fibrosis and cirrhosis. Using in vitro models to understand the molecular pathogenesis has been extremely beneficial; however, most studies have utilized only short-term exposures, highlighting a limitation in current research to model extended fat-induced liver injury" More... | Share Article