Roche货号11745832910现货DIG-High Prime DNA标记及检测启动装I上海睿安生物13611631389

DIG-High Prime DNA标记及检测启动装I

sufficient for 12 labeling reactions，sufficient for 24 blots，suitable for hybridization，suitable for Northern blotting，suitable for Southern blotting

别名:DIG系统，DNA标记

说明

一般描述:

DIG High Prime DNA标记及检测起始试剂盒I使用类固醇半抗原地(gāo)辛(DIG)，通过酶促免疫检测标记DNA探针标记，以便进行杂交和后续显色检测。此试剂盒包含即用型封闭液——由硝基四氮唑蓝(NBT)/5-溴-4-氯-3-吲哚基磷酸酯(BCIP和DIG Easy Hyb颗粒组成的综合储备液。DIG-High Prime混合物包括稳定的Klenow酶、核苷酸、引物和反应缓冲液——所有成分均溶于一种方便的试剂中。样品材料可以是:至少100 bp的DNA片段，线性化质粒，cosmid或λ DNA，或超螺旋DNA“随机结合的”DNA标记方法首先由Feinberg和Vogelstein开发，基于将所有可能序列的寡核苷酸杂交至待标记的变性DNA。初始加入的DNA仅作为标记DNA的合成模板，在反应过程中不会降解，因而可标记极少量的DNA(10ng)。该方法借助Klenow聚合酶，以随机寡核苷酸的3′-OH末端为引物，合成互补DNA链。反应物中存在洋地黄毒苷标记的修饰脱氧核糖核苷三磷酸掺入新合成的互补DNA链中。这种便捷的试剂盒可通过碱不稳定的DIG-11-dUTP随即引发标记DNA模板，显色检测DIG标记杂交产物。我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品为一种安全的化学品。DIG系统是灵敏且具有成本效益的方案，可替代核酸放射性标记和检测。有许多已发表论文证明了DIG系统的通用性，因此，使用放射性标记不再是标记用于杂交的DNA的唯一选择。

应用

DIG-High Prime DNA标记和检测起始试剂盒I已用于多种杂交技术:

• Southern印迹
• Nrthern印迹
• 斑点印迹
• 菌落和噬菌斑杂交
• 所有类型的过滤杂交
• 总基因组DNA的单拷贝基因检测，甚至可检测高度复杂的生物体样品，如人类、大麦和小麦

一小时或过夜孵育后，DIG标记的探针产量很高。

特点和优势:我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品为一种安全的化学品。DIG系统是灵敏且具有成本效益的方案，可替代放射性标记和放射法检测核酸。现有的许多出版物证明了DIG系统的多功能性，因此使用放射性标记不再是杂交DNA标记的唯一选择。

包装:1个试剂盒包含7种组分。

质量:已通过斑点杂交试验测试功能。

其他说明:仅用于生命科学研究。不用于诊断操作。

同行评审论文(部分文献)

An efficient Agrobacterium-mediated transformation system for poplar.Ali Movahedi et al.International journal of molecular sciences, 15(6), 10780-10793 (2014-06-17)Poplar is a model system for the regeneration and genetic transformation of woody plants. To shorten the time required for studies of transgenic poplar, efforts have been made to optimize transformation methods that use Agrobacterium tumefaciens. In this study, anExpression of baculovirus anti-apoptotic genes p35 and op-iap in cotton (Gossypium hirsutum L.) enhances tolerance to verticillium wilt.Juan Tian et al.PloS one, 5(12), e14218-e14218 (2010-12-15)Programmed cell death plays an important role in mediating plant adaptive responses to the environment such as the invasion of pathogens. Verticillium wilt, caused by the necrotrophic pathogen Verticillium dahliae, is a serious vascular disease responsible for great economic lossesCytochrome P450 107U1 is required for sporulation and antibiotic production in Streptomyces coelicolor.Zhenhua Tian et al.Archives of biochemistry and biophysics, 530(2), 101-107 (2013-01-30)The filamentous bacterium Streptomyces coelicolor has a complex life cycle involving the formation of hair-like aerial mycelia on the colony surface, which differentiate into chains of spores. Genes required for the initiation of aerial mycelium formation have been termed 'bld'Screening for recombinants of Crambe abyssynica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration.Weicong Qi et al.Scientific reports, 5, 14033-14033 (2015-09-12)The T-DNA region of pMF1 vector of marker-free system developed by Wageningen UR, has Recombinase R-LBD gene fusion and nptII and codA gene fusion between two recombination sites. After transformation applying dexamethasone (DEX) can activate the recombinase to remove theIdentification and characterization of a Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae) thermotolerance-Related Gene: Bx-HSP90.Feng Wang et al.International journal of molecular sciences, 13(7), 8819-8833 (2012-09-04)Temperatures directly influence the distribution and intensity of pine wilt disease caused by the pine wood nematode, Bursaphelenchus xylophilus. To date, however, little is known about the causation and mechanism of this influence. The molecular chaperone HSP90 is a key